Gynura root has been used extensively in Chinese folk medicine and plays a role in promoting microcirculation and relieving pain. However, its hepatic toxicity should not be neglected. Recently, we admitted a 62-year old female who developed hepatic veno-occlusive disease (HVOD) after ingestion of Gynura root. Only a few articles on HVOD induced by Gynura root have been reported in the literature. It is suspected that pyrrolizidine alkaloids in Gynura root might be responsible for HVOD. In this paper, we report a case of HVOD and review the literature.

Intestinal lymphangiectasia (IL) is a rare disease characterized by dilated lymphatic vessles in the intestinal wall and small bowel mesentery which induce loss of protein and lymphocytes into bowel lumen. Because it most often occurs in the intestine and cannot be detected by upper gastroendoscopy or colonoscopy, and the value of common image examinations such as X-ray and computerized tomography (CT) are limited, the diagnosis of IL is difficult, usually needing the help of surgery. Capsule endoscopy is useful in diagnosing intestinal diseases, such as IL. We here report a case of IL in a female patient who was admitted for the complaint of recurrent edema accompanied with diarrhea and abdominal pain over the last twenty years, and aggravated ten days ago. She was diagnosed by M2A capsule endoscopy as a primary IL and confirmed by surgical and pathological examination.

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Siglec-15 is an important immunosuppressive molecule considered to be a key target in next generation tumor immunotherapy. In this study, we screened 22 high affinity antibodies that specifically recognize human Siglec-15 by using a large human phage antibody library and 5 representative sequences were selected for further study. The results showed the binding activity of 5 antibodies to Siglec-15 (EC 50 ranged from 0.02368μg /mL to 0.07949 μg /mL) and in 2 Siglec-15 overexpressed cell lines, 3 antibodies had the strongest binding activity, so the 2 clones were discarded for further study. Subsequently the affinity of 3 antibodies were measured by Bio-layer interferometry technology (5-9×10E-09M). As the reported ligands of Siglec-15, the binding activity of Siglec-15 and sialyl-Tn, CD44, MAG and LRRC4C can be blocked by 3 of the antibodies. Among these, 3F1 had a competitive advantage. Then, the antibody 3F1 showed an obvious ADCC effect (EC 50 were 0.85 μg/mL). Further, antibody 3F1 can reverse the inhibitory effect of Siglec-15 on lymphocyte proliferation (especially CD4 + T, CD8 + T) and cytokine release (IFN-γ).Given the above results, 3F1 was selected as a candidate for the in vivo pharmacodynamics study. In the tumor model of Balb/c Nude mice, 3F1 (10 mg/kg) showed certain anti-tumor effects (TGI was 31.5%) while the combination of 3F1 (5 mg/kg) and ERBITUX (5 mg/kg) showed significant antitumor effects (TGI was 48.7%) compared with the PBS group. In conclusion, novel human antibody 3F1 has This article has not been copyedited and formatted. The final version may differ from this version.