Multiple displacement amplification (MDA) has become one of the most commonly used method of whole genome amplification (WGA) due to the high processivity, strand displacement capacity and high fidelity of the phi29 DNA polymerase, MDA generate vast amount of DNA with higher molecules weight (up to 100kb) and greater genome coverage. Along with the development of the sequencing platform, it is possible to sequence the MDA-amplified DNA molecules with over 20kb by long-read sequencing. However, one of the challenges is the formation of chimeras, which exist in all MDA products, and seriously interfere with the downstream analysis of the long-read sequencing data of MDA-amplified DNA. In this study, we constructed 3rd-ChimeraMiner, a chimera detection pipeline for analyzing the long-read sequencing of MDA products, recognizing chimeras, and integrating chimeras into the downstream analysis. Five sequencing data of MDA with different magnification fold were analyzed in here, the proportions of chimeras are much higher than that of next-generation sequencing reads and increase with the increase of magnification folds, ranging from 42% to over 76%. After comparing, 99.92% of recognized chimeras have been demonstrated not to exist in original genomes. After detecting chimeras by 3rd-ChimeraMiner, the full-length mapping ratio increased, means more PacBio data could be used in downstream analysis, and mean 97.77% inversions were removed after transferred chimeras into normal reads. 3rd-ChimeraMiner revealed efficiency and accuracy in discovering chimeras from long-read sequencing data of MDA, and is promising to be widely used in single-cell sequencing.

With the development of The Times, the level of science and technology in China has made new breakthroughs. The development of medium-voltage engineering benefits from the innovation of science and technology, and has developed to a new height. At the same time, the simulation technology of medium-voltage non-power outage equipment engineering has also made new breakthroughs, which is widely concerned by the medium-voltage engineering practitioners. Engineering simulation is the key to the quality of medium-voltage engineering, and the engineering simulation technology of medium-voltage non-power off operation equipment directly affects the quality of engineering simulation. The development and innovation of this technology have become an important way for the development of medium-voltage engineering industry. Therefore, this paper firstly gives a brief overview of the engineering simulation technology of medium-voltage non-power outage operation equipment, which is suitable for the background of The Times, and then conducts in-depth research and analysis on the innovative development and future trend of the engineering simulation technology of medium-voltage non-power outage operation equipment, hoping to bring positive influence to the development of medium-voltage engineering industry.

With the continuous development of cloud computing technology in China, cloud computing technology has been widely used in various fields of production. Because there are some defects in traditional automation medium voltage power outage operation equipment and tools, and the application of cloud computing technology in medium voltage power outage operation equipment and tools can effectively improve the production rate of products, thus promoting the development of medium voltage power outage operation equipment and tools. Therefore, this paper first briefly explains the advantages of cloud computing technology, and briefly discusses the application of cloud computing technology in the automatic control of medium voltage non-blackout operation equipment and tools, for readers’ reference.

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The medium and low voltage distribution network system can provide information design according to the needs and usage habits of users, realize a series of service applications based on the management location data provided by the medium and low voltage system, and finally realize the information of distribution network resources. This paper mainly introduces the background and significance of the subject research and the work content and objectives of the subject. Demand analysis mainly introduces the overview of the system and describes the functional requirements; The system design describes the process of system establishment and the function realized finally. The summary part summarizes the development of medium and low voltage technology and the trend of its application in distribution network.