s: We have studied the oscillation behavior of Chinese herb medicine Panax japonicus C. A. Mey. var.major (Burk.) C. Y. Wu et K. M. Feng in oscillation reaction system acetone-KBrO3-MnSO4-H2SO4. Also discussed the effects of concentration of substrate, H2SO4, KBrO3, Mn2+ on the period, amplitude and life of oscillation. The action of each substance in the oscillation system was verified. It provided a new way for herbal medicine identification.

Objective: Optimizing the extraction of total alkaloids from Caulophyllum robustum. Methods: The taspine which is a type of alkaloids of Caulophyllum robustum is selected as the target index. First, developing the HPLC analysis protocol; second, using orthogonal design method to optimize the hydrochloric acid leaching protocol; third, using cation exchange column to isolate the alkaloids and examining the optimal conditions. Results: the optimal conditions of hydrochloric acid leaching protocol is using 7 column volume of 1% hydrochloric acid for 3 times. The materials are loaded onto the LSD001 cation exchange column with optimal pH 4 and flowrate 2ml/mL, the elution buffer is 10 column volume 5% hydrochloric acid. After desalting, the yield of alkaloids is 1.35% which includes 6.83% of taspine.