In this article, the tube formation assays of Figs. 1H, 4F and transwell assays of Figs. 1G, 4E has been corrected. Hence, Figs. 1 and 4 have been replaced.The original article has been corrected.Publisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

This study aimed to study miR-145’s correlation with Th1/Th2 in premature rupture of fetal membranes (pPROM) with nanogold-related PCR. Sixty pregnant women with pPROM between January 2019 to December 2019 were selected as the treatment group, and another sixty healthy pregnant women at the stage were chosen as the control group. Blood samples were collected for the quantification of miR-145 and the Th1/Th2 ratio. The result showed that nanoPCR could detect miR-145 with higher sensitivity and specificity. Then, nanoPCR detected a decreased miR-145 in pPROM pregnant women. Immunofluorescence staining and flow cytometry data presented a higher Th1/Th2 ratio in pPROM pregnant women. Western blotting showed the Th1/Th2 ratio increased significantly in pPROM pregnant women; also, the IFN-γ and IL-2 secreted by Th1 increased at the same time. On the other hand, the IL-4 and IL-6 secreted by Th2 were reduced, consistent with the change in Th2’s pattern in number reduction. A significant negative correlation was shown between Th1, Th1/Th2, and miR-145, and a positive correlation was shown between Th2 and miR-145. In conclusion, we propose thatin pPROM pregnant women, the decrease in miR-145 induces Th1 proliferation and Th2 reduction, causing relative cytokines to change and the immune system to lose imbalance. As more infections occur, and pPROM occurred as a result of fetal membrane fragility.

Purpose To analyze the impact of surgical compliance on ovarian cancer patients’survival and explore the factors affecting surgical compliance. Materials and methods Ovarian cancer patients from 2004-2015 in the SEER database were selected. Clinical, demographic, and treatment characteristics of patients in two groups with good and poor surgical compliance were compared. Kaplan-Meier curves and Cox regression methods were used to analyze the effect of surgical compliance on overall survival (OS) and cancer-specific survival (CSS). Surgical compliance and other independent risk factors were included to construct OS and CSS column-line plots, and the predictive power of the models was assessed using the Harell consistency index (C-index), decision-curve analysis (DCA), subject work characteristics (ROC) curves, and calibration curves. Binary logistic regression was used to identify significant factors affecting surgical compliance. After balancing confounders using propensity score matching (PSM), the effect of surgical compliance on OS and specific survival (CSS) was again assessed. Results A total of 41859 ovarian cancer patients were enrolled in the study, of which 783 (1.87%) belonged to the group of those who were advised to have surgery but the patient refused and had poor surgical compliance, of which 41076 (98.13%) belonged to those who had good compliance in complying with surgical orders. Cox multifactorial analysis showed that surgical compliance was an independent prognostic factor for patients with ovarian cancer. Kaplan - Meier analysis also showed that OS and CSS were significantly better in patients with good compliance than in those with poor compliance ( P < 0.0001). The study data were randomly divided into a training set (n = 29301) and a validation set (n = 12558) according to 7:3, and the column-line plots of OS and CSS were constructed using surgical compliance and independent prognostic factors in the training set and internally validated by the validation set, which determined the superior performance of the nomogram by ROC and DCA curves in comparison with the Grade staging and the AJCC stage system. Time roc, C index, and calibration curves demonstrate the discrimination and calibration of the predictive model. Poor surgical compliance was associated with older age, pathological staging of germ cell type tumors, late staging, presence of local and distant metastases, higher CA-125 values, and lower household income. 712 pairs of participants were enrolled in the study after 1:1 propensity score matching (PSM) to balance confounders, and a Kaplan-Meier analysis also demonstrated that patients with good surgical compliance had significantly better OS and CSS than patients with poor compliance. superior to patients with poor compliance ( P < 0.0001). Conclusion Surgical compliance is an independent prognostic factor predicting OS and CSS in patients with ovarian cancer and is significantly associated with survival. Poor surgical compliance was associated with older age, pathological staging of germ cell type tumors, late staging, presence of local and distant metastases, higher CA-125 values, and lower family income.