Base editing is an emerging genome editing technology with the advantages of precise base corrections, no double-strand DNA breaks, and no need for templates, which provides an alternative treatment option for tumors with point mutations. However, effective nonviral delivery systems for base editors (BEs) are still limited. Herein, a series of poly­(beta-amino esters) (PBAEs) with varying backbones, side chains, and end caps were synthesized to deliver plasmids of BEs and sgRNA. Efficient transfection and base editing were achieved in HEK-293T-sEGFP and U87-MG-sEGFP reporter cell lines by using lead PBAEs, which were superior to PEI and lipo3k. A single intratumor injection of PBAE/pDNA nanoparticles induced the robust conversion of stopped-EGFP into EGFP in mice bearing xenograft glioma tumors, indicating successful gene editing by ABEmax-NG. Overall, these results demonstrated that PBAEs can efficiently deliver BEs for tumor gene editing both in vitro and in vivo.

This paper studies the effect of Mandarin proficiency on poverty reduction and its mechanism. The China General Social Survey (CGSS) is taken as the data source. From the perspective of social integration, the poverty reduction effect of Putonghua and its mechanism are empirically studied from the perspective of social interaction, social fairness, and social trust. The findings showed that improving the ability to listen and speak Mandarin positively affects social interaction, social trust, and social fairness. It showed that Putonghua proficiency positively correlates with the suppression of economic poverty. After the occurrence of poverty, whether a sample of absolute poverty or relative poverty, the ability to listen and speak in Mandarin has a specific inhibitory effect on social interaction, social trust, and social fairness. After the occurrence of poverty, the frequency of social interaction, social trust, and social public since have been reduced to a certain extent. Specifically, the ability to express Mandarin has shown remarkable results in improving social interaction, and the ability to listen among ordinary people has shown remarkable results in enhancing social fairness. The results of this paper provide empirical evidence of poverty alleviation in China via Improving Mandarin Proficiency. This research is also of great significance for optimizing poverty alleviation paths through language in the post-poverty alleviation era.

Based on the Big Five personality trait dimensions of non-cognitive ability measures, the CFPS 2020 database was used as the basis, and the self-answered questionnaires and messages that were under the age of 18 and had information on physical activity were selected as the study sample from the total sample of 28590 in this database, and the valid 1562 samples were used as the study subjects, and the corresponding options and answers were selected as the dependent variables related to the Big Five personality of responsibility, agreeableness, extraversion, and neuroticism. After constructing a model with logit regression and calculating the marginal effects, it was empirically demonstrated that increasing the frequency of physical exercise (frequency) had positive promotion or improvement effects on adolescents' sense of responsibility, agreeableness, extraversion, and neuroticism, with the frequency of physical exercise (frequency) in the past 12 months as the dependent variable, and the effects were different between urban and rural areas, age, and gender. There were differences between urban and rural areas, age, and gender.