No abstract

Lung cancer remains a leading cause of cancer-associated mortality worldwide, however, molecular mechanisms underlying lung cancer tumorigenesis and progression remain unknown. Here, we report evidence showing that one member of the mammalian methyltransferase-like family (METTL), METTL7B, is a potential molecular target for treatment of non-small cell lung cancer (NSCLC). METTL7B expression was elevated in the majority of NSCLC comparing to normal tissues. Increased expression of METTL7B contributed to advanced stages of tumor development and poor survival in NSCLC patients. Lentivirus-mediated shRNA silencing of METTL7B suppressed proliferation and tumorigenesis of cancer cells in vitro and in vivo. Investigation on gene expression profiles of NSCLC cells revealed that abundant cell cycle related genes were downregulated in the absence of METTL7B. Pathway enrichment analysis indicated that METTL7B participated in cell cycle regulation. Notably, CCND1, a key regulator for G1/S transition, was significantly decreased with the depletion of METTL7B, resulting in G0/G1 arrest, indicating that METTL7B is critical for cell cycle progression. Taken together, our findings implicate that METTL7B is essential for NSCLC development and progression. METTL7B might serve as a potential therapeutic target for NSCLC.

No abstract

There exist bounded transmission delay and data packet dropout in the networked control systems (NCSs). When the sensors and actuators are time-driven and controllers are event-driven, the NCSs can be modelled as a class of discrete-time systems with time-varying input delay. Most of similar articles simply combine delay and packet dropout to analyse and synthesise NCSs without distinguishing their different impacts, which leads to conservative results. In this study, the authors summarise that the number of consecutive data packet dropout increases gradually in case of packet dropout. A novel Lyapunov-Krasovskii functional is constructed based on this increment property, so less conservative results are obtained through the Lyapunov-Krasovskii functional approach. In addition, the upper bound of a Lyapunov functional difference cross term is reasonably estimated to further reduce the conservativeness. Stability and stabilisation criteria which are separately related to the transmission delay and data packet dropout are presented. The obtained conditions are based on linear matrix inequalities, which can be solved easily by MATLAB or other numerical software.