Fusarium head blight (FHB) is caused by Fusarium graminearum, which contributes serious economic losses to crop yields, and is an epidemic disease of wheat. The resistance to tebuconazole of 1,118 F. graminearum strains obtained from different areas of Henan Province in 2018–2020 was determined by the discriminatory doses of 5.0 μg/ml. A total of 362 F. graminearum strains resistant to tebuconazole were detected, and the frequency of resistance of F. graminearum strains to tebuconazole was 30.7%. The EC50 and EC90 values of F. graminearum strains to tebuconazole were calculated according to the mycelial growth inhibition of the Henan F. graminearum population. The range of EC50 values of 756 sensitive strains and 362 resistant strains were 0.01–0.69 and 0.02–3.31 μg/ml, and the average ± SD EC50 of sensitive and resistant strains were 0.13 ± 0.01 and 0.40 ± 0.13 μg/ml. The range of EC90 values of 756 sensitive strains and 362 resistant strains were 0.31–4.89 and 0.65–18.69 μg/ml, and the average ± SD EC90 values were 1.13 ± 0.05 and 2.98 ± 0.09 μg/ml. The CYP51A and CYP51B genes of the sensitive and resistant strains were amplified separately by polymerase chain reaction (PCR) and analysed. The results showed that among the 150 resistant strains, only six resistant strains had amino acid mutation, which changed from serine to tyrosine (TCA‐TAT, Ser‐Tyr) at position 169 of CYP51B gene, and no amino acid mutation was found in CYP51B of the other 144 resistant strains. No amino acid mutation was found in the CYP51A gene of 150 resistant strains. These results provide important information to growers for monitoring tebuconazole resistance in F. graminearum field populations from wheat in Henan Province.
With the rapid development of industrialization and urbanization rate, the domestic water consumption in China shows a rapid increasing trend. This paper summarizes the relationship between per capita domestic water consumption, urbanization rate and per capita GDP by studying the domestic water consumption laws of typical countries in the world. The domestic water structure of the United States, France, Germany and Japan were chosen as study case. And we find the similarity of domestic water structure between Japan and China, which means Japan can be used as a sample for analogy prediction. Then, the overall prediction and analogy prediction are used to forecast the domestic water demand in China. The results show that the results of these two methods are consistent. The prediction results can help decision-making departments to make reasonable policies in water resources planning.
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