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Compared with 2D tumor cell culture, 3D tumor cell culture can better simulate the microenvironment of signal transduction between cells and extracellular matrix. As one of the best cell models in tumor research, it has been widely used in the study of cancer cell morphology, nanotechnology drug delivery system, and anticancer drug screening. The main theme of this paper is to review the previous research of 3D cell culture applying to tumors in vitro and the prospects for the applications of 3D cell culture system. Keywords3D Cell Culture System, 2D Cell Culture System, In Vitro Tumors Introduction2-dimensional (2D) culture means a method of culturing tutors by building models outside the body in two dimensions. Regarding as the 3-dimensional (3D) culture, it is also a way to culture tissues but one more dimension than 2D culture. In vitro, tumoris anartificial tissue, is established outside of bodies as a model to culture in vitro tumors for better tumor research.The previous work on the three-dimensional (3D) cell culture system has indicated that the morphology and function of isolated cells in 3D culture are closer to the real growth state of these cells in vivo comparing the traditional two-dimensional (2D) cell culture [1]. Due to the highly abnormal graphic and mechanical constraints compelled on cells in 2D cultures could alter cellular interactions with the extracellular matrices (ECM) and the signaling pathway; 2D cell cultures cannot simulate the cellular properties of normal tissues. In addition, 2D cell culture oversimplifies the various processes that Nano-medicine reaches its targeted site in vivo. # Contributed equally, signature by the alphabetical order of names.

In the 1950s and 1960s, the growth of “Hanshan fever” in the United States may be considered a successful case of Chinese culture “going globally.” The translation and introduction of Hanshan’s poetry from China to the United States have grown in popularity, with Gary Snyder playing a key role. Communication and translation studies are closely related, and the essence of translation can also be considered the dissemination of information in a foreign language setting. The following four dimensions of Snyder’s considerable influence on the translation and introduction of Hanshan’s poetry were explored in this study, based on gatekeeping theory in the communication field, i.e., gathering, filtering, processing, and disseminating of information. It was discovered that Snyder has had a critical part in the translation and introduction of Hanshan’s poetry as a gatekeeper, as well as making significant contributions to their renewal in a foreign setting.

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