Enzymatic hydrolysates from Oysters (OAH) display multiple biological activities. Previously, a 3~5 KDa oyster ultrafiltration component (OUP) showed a high property of preventing skin oxidation. Subsequently, we identified specific peptides with such activity. OUP was fractionated stepwise by Sephadex-G25 and RP-HPLC, and active fractions were screened using UV-irradiated HaCaT cells. The most active fractions (OP5-3) were analyzed by LC-MS/MS and a total of 17 peptides were identified. Results from mass spectrometry showed that OP5-3 consisted of peptides with a molecular weight range of 841.51–1786.92 Da. Six of these peptides were synthesized for validating the activity of resisting skin oxidation in the same cell model. All six peptides showed varying degrees of antioxidant activity, while pretreatment of HaCaT cells with AIVAEVNEAAK alleviated UV cytotoxicity, inhibited metalloproteinase 1 (MMP-1) expression, and showed the highest activity to resist UV-induced skin photo-oxidation among these peptides. In addition, results from molecular docking analysis of MMP-1 with AIVAEVNEAAK showed that AIVAEVNEAAK suppresses its enzymatic activity by directly interacting with MMP-1 and thus exhibit anti-photoaging activity.
While the visible plume from a cooling tower is not a pollutant, it can affect the surrounding environment. Moreover, the accompanied evaporation loss has a great potential for wastewater recovery. In the present study, a novel coupling technology for water conservation and plume abatement was proposed, and its feasibility was verified by using thermodynamic analysis. A surface-type heat exchanger was designed and a thermodynamic calculation model was established. Next, based on the principle of “no plume,” the effect of the number of heat exchanger units (N) and the circulating water volume (G) on the water conservation and plume abatement was evaluated under design condition. Results showed that the optimized parameters for the operation of the cooling towers were N = 8 and G < 3000 m3/h, which have a good effect on water conservation and plume abatement. Furthermore, as per the condensation calculation model, the average water conservation amount was 1.105 kg/s and the annual water conservation amount reached 2.8641 × 107 kg.
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