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Purpose: Lactic acid metabolism may play an imperative role in the development of Retinopathy of Prematurity (ROP). Through bioinformatics analysis, we strive to identify the potential lactate metabolism-related genes (LMRGs) of ROP, and predict their functions and internal mechanisms. Methods: GSE158799 microarray dataset is located on the GPL18635 platform (Ion Torrent Proton), which is from the National Center for Biotechnology Information (NCBI). Firstly, we used R software to screen for potentially differentially expressed LMRGs related to ROP. Then we analyzed the differentially expressed genes (DEGs) by protein–protein interactions (PPI), correlation analysis, gene-ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, Gene Set Enrichment Analysis (GSEA), and assessment of immune cell infiltration. Results: A total of 41 differentially expressed LMRGs were identified among 284 genes in 3 P17_OIR mice and 3 P17_OIR mice. The results of PPI analysis indicated the interaction among these LMRGs. And the GO and KEGG analysis revealed several enriched pathways related to lactate metabolism, such as PI3K-Akt signaling pathway and Apelin signaling pathway. Moreover, immune infiltration analysis showed that the expression levels of immune cells in the sample changed greatly, especially M1 and M2 macrophages. Conclusion: We identified 41 potential LMRGs correlated to ROP. These DEGs may regulate lactate metabolism, leading to the changes of metabolism and immunity, thereby inducing the development of ROP. Our results will expand our understanding of the intrinsic mechanism of ROP and may be helpful for the directions for treatment of ROP in the future.

To solve the problem of silicide coatings on tantalum substrates failing due to elemental diffusion under high-temperature oxidation environments and to find diffusion barrier materials with excellent effects of impeding Si elemental spreading, TaB2 and TaC coatings were prepared on tantalum substrates by the encapsulation and infiltration methods, respectively. Through orthogonal experimental analysis of the raw material powder ratio and pack cementation temperature, the best experimental parameters for the preparation of TaB2 coatings were selected: powder ratio (NaF:B:Al2O3 = 2.5:1:96.5 (wt.%)) and pack cementation temperature (1050 °C). After diffusion treatment at 1200 °C for 2 h, the thickness change rate of the Si diffusion layer prepared using this process was 30.48%, which is lower than that of non-diffusion coating (36.39%). In addition, the physical and tissue morphological changes of TaC and TaB2 coatings after siliconizing treatment and thermal diffusion treatment were compared. The results prove that TaB2 is a more suitable candidate material for the diffusion barrier layer of silicide coatings on tantalum substrates.

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Retinopathy of Prematurity (ROP) is a multifactorial disease characterized by abnormal retinal vascular growth in premature infants, which is one of the leading causes of childhood blindness. Lactic acid metabolism may play an imperative role in the development of ROP, but there are still few relevant studies. Our team use a dataset GSE158799 contained 284 genes in 3 P17_OIR mice and 3 P30_OIR mice to identify 41 potentially differentially expressed lactate metabolism-related genes (LMRGs) related to ROP. Then through bioinformatics analysis, we strive to reveal the interaction, the enriched pathways and the immune cell infiltration among these LMRGs, and predict their functions and internal mechanisms. These DEGs may regulate lactate metabolism, leading to the changes of metabolism and immunity, thereby inducing the development of ROP. Our results will expand our understanding of the intrinsic mechanism of ROP and may be helpful for the directions for treatment of ROP in the future.