Materials and reagents. A Sylgard 184 poly(dimethylsiloxane) (PDMS) kit was purchased from Dow Corning Co. (Midland, MI, USA). Glass plates coated with chromium and photoresist for chip fabrication were obtained from Shaoguang Microelectronics Corp. (Hunan, China). All the other chemicals were of analytical grade and used without further purification. Milli-Q grade water (Millipore Inc., Bedford, MA, USA) was used for preparing all solutions and cleaning microchannels. 10 mM phosphate-buffered saline (PBS, pH 7.4) containing 137 mmol/L NaCl, 2.7 mmol/L KCl, 8.72 mmol/L Na 2 HPO 4 , and 1.41 mmol/L KH 2 PO 4 . Glass capillary (1.0 mm id, 10 cm length) was purchased from Sichuan University Inc. (Chengdu, China). Free hemoglobin test kit was acquired from Jiancheng Bioengineering Institute (Nanjing, China). Silicone tube was from Nuoyawei Inc. (Shenzhen, China). Theophylline was from National Institutes for Food and Drug Control (Beijing, China). Other chemicals were of analytical grade and used without purification.

Gastrodia elata Bl. (Orchidaceae) is an important traditional medicinal plant as well as a famous foodstuff in China. In the present article, the HPLC chromatograms of different preparation processes were reported, and structures of nine phenolic compounds, isolated from Gastrodia elata, were assigned. The isolated compounds were identified as 1,3-bis(4-hydroxybenzyl)citrate (1), gastrodin (2), 4-hydroxybenzyl alcohol (3), 1-(4-beta-D-glucopyranosyloxybenzyl)citrate (4), 4-hydroxybenzaldehyde (5), parishin B (6), 4-hydroxybenzyl methyl ether (7), 4-hydroxybenzyl ethyl ether (8), and 4-(4'-hydroxybenzyl)phenol (9). Compounds 1 and 4, named as parishin D and E, were new. Their structures were elucidated on the basis of spectral analyses, including 2D NMR spectroscopy.

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