The Podocarpus Nagi kernel oil was prepared and its chemical constituents were confirmed accordingly in this work. The constituents and the contents of the obtained oil were identified as: total flavonoids (4.2%, calculated according to the rutin), (9Z,12Z)-9,12-octadecadienoic acid(38.2%), arachidic acid(0.11%), cis-11-eicosenoic acid(1.40%), cis-11,14-eicosadienoic acid(8.04%), -3(0.2%), -6(48.0%), -9(21.0%), vitamin E(2.11mg/100g). The microelements in this oil was also tested using ICP-MS and the results exhibited that this oil contains numbers of microelements beneficial for human health: such as V(0.006µg/g), Cr(0.024µg/g), Mn(40.109µg/g), Fe(2.292µg/g), Co(0.007µg/g), Zn(4.316µg/g), As(0.009µg/g), Se(0.240µg/g), Sr(0.453µg/g).Then, the oil was evaluated for its in vitro antioxidant effect against DPPH[?], which exhibited noteworthy scavenging ability against DPPH [?]. In addition, the oil was also evaluated for its preliminary in vitro anticancer activity against four cancer cell lines: the results showed that it exhibited the highest inhibition against gastric cancer, breast cancer (MCF-7), lung cancer(A549) and Hela cell lines with the highest inhibitions of 64.30%±2.80, 52.87%±2.57, 93.21%±2.24 and 88.33%±2.08 at the concentrations of 25mg/mL, 25mg/mL, 50mg/mL and 50mg/mL, and with the IC50s of 48.47mg/mL, 763.14mg/mL, 11.47mg/mL and 23.77mg/mL respectively. These findings demonstrated that this oil can be regarded as the functional edible oil by comparing the contents of edible oils used in the field of food.
