This paper shows the potential of phloroglucinol for In Vitro regeneration and tuberization of microshoots of Tinospora cordifolia. Within a week of nodal explants culture, axillary bud in nodal segments sprouted in all the media investigated including basal MS media without any growth regulators. Maximum response (52.2 per cent) evinced by MS media supplemented with 6.98 µM Kin. The same media produced maximum response for multiple shoot formation out of total shoot bud sprouted fostering average 3 shoots with 3.9 cm shoot length and 4.2 number of leaves per shoot. Effect of PG also investigated for mass multiplication and In Vitro rooting. Basal MS + 6.98 µM Kin + 79.4 µM PG increased shoot bud induction from 52.2 per cent to 84.8 per cent and multiple shoot production per cent from 12.9 to as high as 60.3 percent and also supported maximum number of shoot per explants (7.5). The same treatment promoted highest axillary shoot proliferation in terms of shoot length (3.9 cm) and number of leaves per shoot (4.3). Phloglucinol induced In Vitro tuberization in microshoots. Maximum rooting response observed in ½ MS medium supplemented with 7.4 µM IBA and 793.7 µM PG with 81.1 culture responding taking just 8.7 days to sprout and producing maximum number of roots (3.2) with an average length (3.1 cm). Rooted explants successfully hardened in Soil: Vermicompost: Vermiculite (1:1:1) with 100% success.