A novel recognition element has been synthesized using glycopolymer-modified gold nanoparticles (GNPs) in sugar ratios of 0, 6, 12, and 50%. The as-synthesized glycopolymer-modified GNPs have been employed in a novel lateral flow assay for the detection of proteins. A test solution of concanavalin A (ConA) at 0.01–100 µg mL−1 was readily detectable with the glycopolymer-modified GNPs with a sugar ratio of 6%. The results were clear enough to be visible with the naked eye, which demonstrates the convenience of this lateral flow assay for performing the detection of Shiga toxins and influenza viruses. Therefore, this lateral flow assay provides an attractive biosensor for the detection of proteins without the handling of toxic reagents, while allowing an easy and rapid procedure.

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