In order to explore the change rule of physiological indexes during the aging process of the liquid spawn of Auricularia auricula, this test chooses three species of Auricularia auricula(Hei29, Heiweibanjin and Heiweidanpian), which treasured the activities of antioxidant enzymes, antioxidant substances, membrane peroxidation degree and reactive oxygen concentration during different mycelia age. The conclusion showed that: at the same mycelial age, the vitality of Heiweidanpian was the strongest, Hei29 followed, and Heiweibanjin was the weakest; with the increase of mycelia age, Catalase (CAT) activity, Ascorbic Acid(ASA) content, Malondialdehyde (MDA) content and O2- production rate of the three kinds of Auricularia auricula increased continuously. Among them, ASA content, MDA content and O2- production rate increased significantly, while Superoxide Dismutase (SOD) activity decreased significantly; although the Proline (Pro) content and electrical conductivity of the three kinds of Auricularia auricula showed different trends, which decreased significantly at 14 to 16d and increased significantly at 8 to 10d, respectively, and H2O2 content increased significantly at 12 to 16d. It can be seen that SOD activity, CAT activity, ASA content, MDA content and O2- production rate have significant regular changes during the aging process of the liquid spawn of Auricularia auricula. The quantitative analysis found that when the MDA content is 1.42~1.72 μmol·g-1, the H2O2 content is 3.4~4.3 μmol·g-1 or the O2- production rate is 9.5~12.1 μmol·g-1·min-1, it can be considered Auricularia auricula just started to age or the aging degree is relatively low.

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Because goats grow fast and produce a lot of meat and milk, interest in raising goats is increasing day by day. Especially here, it is very important to do scientific specification management for goat kids. Due to the failure of scientific specification management of the goat kids, the rate of death in the early stages of growth development is more than 20%, and it often does not guarantee the standard body weight of the candidate goat by affecting the pre- and post-natal stages. In particular, it is important to scientifically establish the colostrum recycling technology in the early stages of development, as newborn goats do not have the physiological function of producing and recycling the immune system on their own. Paper introduced a new method for evaluating the level of lactic acidity that easily tests serum Ig in field conditions in newborn goat kids, and newly assessed the biological relationship between the serum level and the ability to grow.

Liquid spawn, an important technical support for industrial production of edible fungi, has some advantages, such as fast growth, strong activity, uniform quality and easy inoculation. However，the study of the fermentation parameters of Pleurotus eryngii liquid spawn is less, which cannot effectively guide the production practice. Therefore, in order to provide a theoretical basis for the production of liquid spawn and industrial production of Pleurotus eryngii, response surface analysis was used to optimize the liquid spawn formula and conditions. The best carbon and nitrogen source of liquid medium was glucose and yeast extract, respectively. The optimum formula of medium was obtained by response surface analysis. Combined with the practical operation, the formula was modified to 29.00 g/L glucose, 2.90g/L yeast extract, 0.90g/L KH2PO4 and 1.00g/L MgSO4. The effects of various factors on the dry weight of mycelia were analyzed, the results indicated that the influence follow the sequence: glucose, KH2PO4, yeast extract and MgSO4, among them, the first two factors had significant effect. The interaction between glucose and KH2PO4 had obviously effects on the dry weight of mycelia. The interaction between glucose and yeast extract had a certain effect. The optimum culture conditions of liquid spawn were obtained by response surface analysis. Combined with the actual situation, the conditions were modified to liquid volume 106.00mL/250mL, rotating speed 165.00r/min, temperature 23.60℃, initial pH 6.70. The effects of various factors on the dry weight of mycelia was analyzed, the results indicated that the influence follow the sequence: initial pH, liquid volume, temperature and rotating speed, among them, the first three factors had extremely significant effect. The interaction of liquid volume and rotating speed, temperature and initial pH had obviously effects on mycelial dry weight.

A foot and mouth disease (FMD) is an acute, febrile and high contagious viral disease in many cloven hoofed domestic animals and more than 70 wild ones, resulting in severe finantial loss throughout the world. Choosing the correct seeds for foot-and-mouth disease (FMD) is an important issue in protecting this disease, and it is urgently necessary to establish a plan for vaccination in areas affected by FMD and to protect new foot-and-mouth disease. The genetic diversity and antigenic diversity of foot-and-mouth disease virus (FMDV) make eradication through vaccination difficult. Variable antigenic types exist in different geographic areas, and research projects on existing antigenic types are necessary to select vaccine in such an environment. From this, in this paper, oligonucleotide primers for detection and Selecting Serotype of FMDV were newly designed and synthesized. In addition, the nucleotide sequence of the 1D gene was aligned, the mutation region was determined, and the homology and phylogenetic relationship of the nucleotide sequence were analyzed. The antigenicity of the FMDV type O strains in the Democratic People's Republic of Korea and the correspondence between them were examined. The neutralization reaction was used to examine antigenicity between FMDV to select waxy seeds. To prevent FMD through this primer design and experimental method, the nucleic acid of FMDV was amplified by RT-PCR. Then, the nucleotide sequence of the 1D gene corresponding to the virus VP1 protein was analyzed and compared to select a seed vaccine strain.