The purpose of this study was to analyze for resonant frequency, vibration transmissibility and damping ratio of multi-layered corrugated structures using a random vibration test. Methods: The random vibration test was performed by the ASTM D4728 specifications using two paperboards (S120, K180) and two types of flutes (A/F, B/F). Damping ratio of the multi-layered corrugated structures was estimated using a theoretical equation derived from the measured resonant frequency and transmissibility. Results: The resonant frequency and vibration transmissibility of the multi-layered corrugated structures of K180 and B-flute were higher than those of S120 and A-flute, respectively; however, the damping ratio of each sample had the opposite tendency. The resonant frequency was inversely proportional to the sample thickness and static stress; vibration transmissibility and damping ratio were not correlated with sample thickness and static stress. In addition, we developed a mathematical model of the resonant frequency with variables of sample thickness and static stress. Conclusions: Results of this study can be useful for environment-friendly and optimal packaging design since vibration has been a key factor in cushioning packaging design.