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Purpose: This research was conducted to study the hygiene level of beauty shop industry and to provide data for improving the environmental hygiene of beauty shops and developing sanitizers by identifying the contaminated microorganisms from hair dust in the clippers promptly. Methods: This survey targeting 248 beauty shop workers in Gyeonggi province was carried out, and hair dust in the clippers from 50 beauty shops was collected. Additionally, microorganisms were identified by using the MALDI-TOF identification method. The SPSSWIN (Ver. 19.0K, SPSS Inc., USA) was used for statistical processing. Results: For the hygiene education in the beauty shop, 50.4% answered 'Yes' and for the sterilization education for each apparatus type, 64.9% answered 'Yes'. For the necessity for hygiene education, 93.3% of respondents with education level of graduate school graduation or higher answered 'It is necessary' and for continuous working with skin disease, 51.6% of the respondents answered 'Yes'. For clipper sterilization, 35.9% answered 'Once a day', and for the reason of no clipper sterilization, 35.0% answered 'Inconvenience due to frequent usage'. The microorganism identified from the dust in the clipper with the MALDI-TOF MS method was Escherichia coli AKD00698 of which the score was 208.0. Conclusion: The results of this research showed that it is necessary to educate beauty shop workers about the hygiene management. Moreover, it is very important significance in the view of hygiene. Because we are able to predict the contamination possibility of other bacteria.