Accumulating pesticide (and herbicide) residues in soils have become a serious environmental problem. This study focused on identifying the removal of two widely used pesticides, isoproturon (IPU) and acetochlor (ACT), by a genetically developed paddy (or rice) plant overexpressing an uncharacterized glycosyltransferase (IRGT1). IRGT1 conferred plant resistance to isoproturon–acetochlor, which was manifested by attenuated cellular injury and alleviated toxicity of rice under isoproturon–acetochlor stress. A short-term study showed that IRGT1-transformed lines removed 33.3–48.3% of isoproturon and 39.8–53.5% of acetochlor from the growth medium, with only 59.5–72.1 and 58.9–70.4% of the isoproturon and acetochlor remaining in the plants compared with the levels in untransformed rice. This phenotype was confirmed by IRGT1-expression in yeast (Pichia pastoris) which grew better and contained less isoproturon–acetochlor than the control cells. A long-term study showed that isoproturon–acetochlor concentrations at all developmental stages were significantly lower in the transformed rice, which contain only 59.3–69.2% (isoproturon) and 51.7–57.4% (acetochlor) of the levels in wild type. In contrast, UPLC-Q-TOF-MS/MS analysis revealed that more isoproturon–acetochlor metabolites were detected in the transformed rice. Sixteen metabolites of isoproturon and 19 metabolites of acetochlor were characterized in rice for Phase I reactions, and 9 isoproturon and 13 acetochlor conjugates were characterized for Phase II reactions in rice; of these, 7 isoproturon and 6 acetochlor metabolites and conjugates were reported in plants for the first time.

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Metal smelting process all kinds of element content directly influence the metal performance index and smelting process control .The amount of carbon become mainly alloy index for divided type and number. LIPS using high power laser ablation material produce plasma, in plasma radiation spectrum analysis; it could be used in the composition of solid, liquid and gas composition and concentration measurement. But the results of the measurement will be affected by some factors, this article through some experiments, that show the Influence factors for the measured results such as the delay time，the different environment of gas and the laser pulse energy and analyzed them.