Background/purpose Autophagy, a lysosome-based degradation pathway that is reportedly activated by mechanical stress and nutrient deprivation, plays an important role in various physiological and pathological events. The present study investigated the level of autophagy and tumor necrosis factor-α(TNF-α) expression in the periodontal ligaments (PDLs) of Sprague–Dawley (SD) rats to analyze the involvement of autophagy and inflammatory cytokines in orthodontic tooth movement (OTM) and maintaining periodontal tissue homeostasis. Materials and methods SD rats (n = 100) were randomly divided into a control group (n = 10) and an experimental group (n = 90). An orthodontic appliance was placed in each rat in the experimental group, and 10 rats were randomly euthanized 15 min, 30 min, 1 h, 2 h, 4 h, 12 h, 1 d, 3 d and 7 d after mechanical loading. The OTM distance was then measured. Hematoxylin and eosin (HE) staining was used to analyze the morphology of the PDL. Immunohistochemical (IHC) staining and tartrate-resistant acid phosphatase (TRAP) staining were also performed. Results After the application of orthodontic force and under the dual effects of mechanical force and starvation caused by compressed vessels, the level of autophagy and TNF-α expression in the PDL fluctuated and exhibited a similar trend. Conclusion Our data suggest a significant correlation between the initiation of autophagy and TNF-α expression, which both exerted positive effects on PDL remodeling during OTM in rats.

Background To investigate the effect of combined oral contraceptives (COC) on orthodontic tooth movement (OTM) and periodontal remodelling in a female rat model. Methods A total of 80 three-month-old female, Sprague-Dawley rats were randomly divided into experimental and control groups. The maxillary first molars were moved mesially using nickel-titanium coil springs (50 g force). The experimental group (N = 40) ingested 1.5 ml COC (Marvelon, 0.12 mg/d, N.V. Organon, Oss, The Netherlands) daily. The control group (N = 40) ingested 1.5 ml saline (0.9% sodium chloride) daily. After 7, 14, 21 and 28 days of force application, 10 rats in each group were euthanased and a vernier calliper was used to measure the orthodontic movement of the first molar. Root resorption at pressure areas was assessed by H and E staining. Micro-CT was used to detect alveolar bone mineral density. Results and conclusion The amount of OTM in the experimental group (0.46 ± 0.16 mm) was significantly less than in the control group (0.85 ± 0.25 mm; p = 0.003) during the 28 days of observation. There were significantly smaller (p = 0.002) root resorption lacunae in the experimental group (111710 ± 4037 pixels) compared with the control group (204962 ± 21318 pixels) after 28 days. There was no statistically significant difference in the bone mineral density between the experimental and control groups throughout the study period (p > 0.05 at each time point). The short-term administration of COC may retard tooth movement and reduce the level of root resorption during OTM in female rats.