Objectives Recently, increasing attention has been concentrated on decrypting the potential of long non‐coding RNAs (lncRNAs) in influencing the progression of human tumors, oral squamous cell carcinoma (OSCC) included. The role of a novel lncRNA, forkhead box D1 antisense RNA 1 (FOXD1‐AS1), has been discussed in multiple cancers. Nevertheless, its function and relevant mechanism in OSCC have been not probed yet. Materials and methods FOXD1‐AS1 expression was detected via RT‐qPCR. Colony formation, EdU, transwell and Western blot analyses tested the functional role of FOXD1‐AS1 in OSCC cells. The relationship between RNAs was assessed by a series of mechanical assays. Results FOXD1‐AS1 was expressed at a high level in head and neck squamous cell carcinoma (HNSC). Knockdown of FOXD1‐AS1 exerted repressive impacts on OSCC cell proliferation, migration, invasion, and EMT. Moreover, FOXD1‐AS1 positively regulated its nearby gene FOXD1 via interacting with miR‐369‐3p. In addition, adenosine deaminase RNA specific (ADAR), known as a RNA‐binding protein (RBP), was capable to bind with FOXD1‐AS1 and FOXD1 simultaneously, and could regulate the stability of FOXD1 mRNA. Aside from that, rescue assays delineated that FOXD1‐AS1 promoted OSCC progression via upregulating FOXD1. Conclusions FOXD1‐AS1 elevates FOXD1 expression to promote OSCC malignant phenotypes through miR‐369‐3p and ADAR.

Background The mandibular second molars demonstrate variations on root and canal morphology. The aim of this study was to investigate all the root canal morphology of mandibular second molars and analyze the morphological variations in patients by gender and age in a Chinese population use CBCT imaging. Methods Cone-beam computed tomographic images of 1200 bilateral mandibular second molars were obtained from 600 patients (300 females and 300 males) who required a preoperative assessment for implant surgery, surgical removal of impacted teeth, orthodontic treatment, surgery of maxillofacial tumour and cysts or LeFort I osteotomy. CBCT images were divided into 5 groups according to age: “15–24 years”, “25–34 years”, “35–44 years”, “45–54 years” and “≥ 55 years”; and 2 groups by gender: “females” and “males”. The following information were recorded: the number of roots and canals and their morphology, the frequency and configuration of C-shaped canals by gender, age and position (left and right). The chi-square test was used to analyse differences between groups. P value of < 0.05 was considered statistically significant. Results Of the 1200 teeth, 61% had two separate roots located mesiodistally, 35.6% had one C-shaped root. The 45.3% teeth had three canals in two-rooted mandibular second molars. The mesial root showed a Vertucci type II configuration in 28.9% cases followed by type IV(24.4%). While the distal root showed a significant higher prevalence of type I configuration in 95.6%. In the examined 1200 teeth, 430 teeth (35.8%) had C-shaped root canals. The prevalence of C-shaped root canal systems was significantly higher in females (42.5%) than in males (29.1%) (P = 0.000), and did not differ with age (P = 0.126). The 80.4% C-shaped canals were bilateral (P = 0.000) and did not differ with side (left and right) (P = 0.758). Conclusions The most commonly observed root morphology for the mandibular second molars was 2 separate roots with three canals.The prevalence of C-shaped root canal is 35.8% and is more higher in females than in males.

IntroductionThe effects of preeclampsia superimposed on chronic hypertension (CHTN-PE) on the structure and function of the human brain are mostly unknown. The purpose of this study was to examine altered gray matter volume (GMV) and its correlation with cognitive function in pregnant healthy women, healthy non-pregnant individuals, and CHTN-PE patients.MethodsTwenty-five CHTN-PE patients, thirty-five pregnant healthy controls (PHC) and thirty-five non-pregnant healthy controls (NPHC) were included in this study and underwent cognitive assessment testing. A voxel-based morphometry (VBM) approach was applied to investigate variations in brain GMV among the three groups. Pearson’s correlations between mean GMV and the Stroop color-word test (SCWT) scores were calculated.ResultsCompared with the NPHC group, the PHC and CHTN-PE groups showed significantly decreased GMV in a cluster of the right middle temporal gyrus (MTG), and the GMV decrease was more significant in the CHTN-PE group. There were significant differences in the Montreal Cognitive Assessment (MoCA) and Stroop word scores among the three groups. Notably, the mean GMV values in the right MTG cluster were not only significantly negatively correlated with Stroop word and Stroop color scores but also significantly distinguished CHTN-PE patients from the NPHC and PHC groups in receiver operating characteristic curve analysis.DiscussionPregnancy may cause a decrease in local GMV in the right MTG, and the GMV decrease is more significant in CHTN-PE patients. The right MTG affects multiple cognitive functions, and combined with the SCWT scores, it may explain the decline in speech motor function and cognitive flexibility in CHTN-PE patients.

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The aim of this study was to investigate root canal curvature and direction of maxillary lateral incisors in Shandong, China. Cone beam computed tomography (CBCT) images of 176 maxillary lateral incisors of 88 patients were collected in Shandong Province, China. Software included with CBCT was used to measure the angle of root canal curvature of maxillary lateral incisors on the maximum bending plane. In addition, the direction of each root canal was recorded. The data were statistically analyzed by SPSS 17.0 software package. The results showed that all the samples had a single canal (Vertucci's type I). The incidence of straight root canals, curved root canals, and S-type root canals was 39.2%, 58%, and 2.8%, respectively. The difference in the mean angle of root canal curvature failed to identify any differences between the left and right side ( P > .05). The most curved root canal of maxillary lateral incisors oriented in the palato-distal direction. The maxillary lateral incisors were mainly curved root canals of which the proportion of moderate curvature was the largest. Software included with CBCT would provide some valuable information for root canal instrumentation of maxillary lateral incisors.