Hydrophilic interaction liquid chromatography (HILIC) is a significant enrichment strategy in glycoproteomics profiling. In this report, hydrophilic magnetic mesoporous silica materials (denoted as FeO@mSiO-IDA) were designed and synthesized as an outstanding enrichment platform for glycopeptide analysis. By taking advantage of their merits, such as large surface area, excellent hydrophilicity and unbiased affinity toward all types of glycopeptides, the FeO@mSiO-IDA nanomaterials were successfully applied to capture glycopeptides from complex samples. A total of 25 glycopeptides from horseradish peroxidase (HRP) digests and 33 glycopeptides from IgG were identified, respectively. Especially, as a result, 424 glycopeptides assigned to 140 glycoproteins were identified from only 2 μL human serum.

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A very simple and amazing approach was proposed to synthesize MPBA functionalized magnetic nanoparticles via the interaction between Fe and SH, and the as-prepared nanoparticles were successfully applied for the efficient analysis of glycopeptides in complex bio-samples with sensitivity and selectivity.

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