Summary Various scientific researches reveals that blood donated by non-remunerated blood donors is safer is many aspects. That is why identifying motivational factors affecting blood donation and recruitment of safe and low-risk donors is a challenge all around the world. Many researches were done in order to identify motivation for blood donating. But research do not study how motivation tools influence and affects motivation and retention of voluntary non-remunerated blood donors and there is a lack of scientific evidence how legislation regulation and different motivation tools applied really influence and affect motivation of voluntary, non-remunerated blood donors. Analysing the situation of blood donation in Lithuania and the experience of motivating donors could help find the connections between the decisions made and proven. The display of these connections is important because it could help to identify the most important factors for the improvement of an object researched as well as create possibilities to purposefully act, fulfil expedient intervention into the processes of legislation regulation and motivation of blood donors in order to seek to improve them and achieve better results in voluntary,