No abstract

The objective of this review was to carry out a scientific systematization on the logistics of soybean chain in Brazil, focusing on losses during harvest, storage, and transport of soy, to demonstrate the economic impacts of losses in the soybean chain. The survey of works related to the theme was conducted in the bases Scopus, SciELO, and in the CAPES portal. In the development of the review, 10 articles were used about the logistics of soybeans in Brazil, 22 studies related to losses in the pre-harvest and soybean harvest, 22 involving soybean transportation and storage losses. The organization of the Brazilian soybean chain is complex due to soybean expansion and the territorial extension of Brazil. The pre-harvest and harvest losses are well explained, but significant losses still occur due to lack of regulation in the harvesters, which can be solved through the training of the operators. Concerning losses during transport and storage, studies are needed to quantify quantitative losses, as these losses may be determinant for the rural entrepreneur's profitability, as well as for the Brazilian competitiveness in the international market in terms of costs.

Bacillus cereus is a gram positive bacterium with sporulation capacity. Here, we report the complete genome sequence of two native B. cereus strains (#25 and #29) isolated from intestinal tract of the crab Ucides sp. from Pacoti River in the State of Ceará, Brazil. The findings of this study might increase the molecular information for Bacillus strains. The data can be used in comparative analyses, origin and distribution, as well support for genetic engineering.

This work aimed to perform the genetic manipulation of the native B. cereus #25 strain and to evaluate the colonization in the intestinal tract of Litopenaeus vannamei. The expression of the GFPmut1, absence of amylase activity, and growth in spectinomycin plates confirmed the transformation of the strain (BC25GM). In the shrimp bioassay, its addiction in the feed showed non-difference for water quality, growth performance, and toxic effects for L. vannamei (survival 100%). The presence of cells expressing GFPmut1 in the midgut of the animals was observed, suggesting that the genetically engineered strain colonized the shrimp intestines. The relative fluorescence was 35% higher in the midgut of the shrimps feed with BC25GM. In conclusion, the present work showed that it is possible to manipulate a native B. cereus strain with the nonspecific pSG1154 plasmid. We reported the possibility to implement native strains as a biotechnology tool applied in aquaculture, especially to deliver beneficial molecules for shrimps.

As análises da relação entre características biométricas em juvenis de robalo-flecha (Centropomus undecimalis) cultivados proporcionam dados importantes para avaliação da biologia e do crescimento, entretanto, mais estudos são necessários nessa área. Dessa forma, esse trabalho teve como objetivo investigar em juvenis de robalo-flecha cultivados, a relação entre o peso vivo com a distância pré-dorsal, largura, comprimento da nadadeira caudal, altura do pedúnculo caudal, altura, comprimento da cabeça, comprimento padrão e comprimento total, identificando o tipo de crescimento envolvido nessa relação. As características comprimento total, comprimento padrão, altura e comprimento da cabeça, apresentaram alta correlação com o peso vivo variando entre 0,92 e 0,94. O tipo de crescimento entre as características relacionadas foi alométrico positivo (b > 3). Os resultados obtidos desse estudo proporcionam informações para ampliar o entendimento na avaliação do crescimento do robalo-flecha em cativeiro, bem como contribuições em estudos realizados com outras espécies e com a aquicultura.