SYNOPSIS A simple method is described for the automated determination of serum iron and tota iron-binding capacity, which eliminates the need to render apparatus or reagents iron-free. The method is faster than normal procedures and its accuracy is comparable with other accepted methods for the determination of serum iron.The available methods for the determination of serum iron are time-consuming and require iron-free glassware. A method which avoids these drawbacks of the conventional serum iron methods and greatly simplifies the determination of serum iron is described here. By using standard components of the AutoAnalyzer system of Technicon Instruments Company Ltd., it is possible to determine both serum iron and total iron-binding capacity colorimetrically without special precautions to make either the apparatus or reagents iron-free. 2,4,6-Tripyridyl-1,3,5-triazine is used as colour-developing reagent because of the high molecular coefficient of absorption of its complex with iron in water (Diehl and Smith, 1960). In spite of the simplicity of the method the results compare very favourably with non-automated methods using both tripyridyl and tripyridyl-triazine as complexing agents.

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