Bovine babesiosis is one of the most important parasitic diseases in the world, it is caused by protozoa of Babesia genus and generates anemia, anorexia, weight loss, jaundice and hemoglobinuria generating loss in production and even the death of the animal. Serological tests are relatively inexpensive and efficient to detect the parasite, but the results depend on factors inherent to the animal and the state of infection. Molecular markers are more precise tools to detect the parasite. The aim of the current systematic review was to review original studies, published between 2010 and 2018, in which molecular techniques were implemented to detect the parasite. The research was carried out using PubMed, LILACS, SciELO and Science Direct databases. 196 records were identified according to the research criteria and only 47 were analyzed. The main molecular techniques reported include Polymerase chain reaction (PCR), nested PCR (nPCR) and semi nested PCR, Real time PCR (qPCR), Reverse ...