Pancreatitis is a common disease in dogs that can be classified as acute or chronic. 1,2 Due to the large variation in clinical signs, the diagnosis of the disorder remains challenging as it may mimic other diseases. 3,4 Measurement of serum or plasma canine pancreatic lipase (cPL), an enzyme that is synthesized in pancreatic acinar cells, is recommended as part of the diagnostic work-up in dogs with suspected pancreatitis. 2,5,6 A validated canine-specific cPL ELISA (Spec cPL) is available at referral laboratories, but because acute pancreatitis is a highly dynamic process that can lead to life-threatening complications quickly, an in-house cPLI test would be valuable for obtaining rapid test results. 2,4,7,8 The aim of this study was to evaluate a point-of-care cPLI test, Vcheck cPL (BioNote, Inc., Hwaseong-si, Gyeonggi-do, Republic of Korea), by performing the following studies: (a) precision, (b) linearity under dilution, (c) comparison to a previously validated cPLI ELISA, Spec cPL, and (d) investigating the performance of the assay in a clinical setting by a retrospective clinical study where cPLI concentrations from dogs with and without acute pancreatitis were determined. In the latter study, it was