This study looked at the online learning readiness and 21st century pedagogical skills among pre-service teachers of a state university in the Philippines during the school year 2021-2022. The descriptive-correlational method was applied through an adapted survey questionnaire administered to 28 pre-service teachers. The findings revealed that pre-service teachers were ready for online learning in terms of computer/internet self-efficacy, self-directed learning, learner control, motivation for learning, and online communication self-efficacy. They were also equipped with 21st century pedagogical skills such as information and communication technology skills, life-long learning skills, flexibility skills, creative problem-solving skills, and critical thinking skills. The findings also indicated a substantial relationship between the pre-service teachers’ readiness in online learning and their 21st century pedagogical skills. The study suggests that universities may create appropriate provisions to provide their faculty members with the necessary professional development training opportunities to raise awareness and improve e-pedagogical skills.