No abstract

In euryhaline teleosts, osmoregulation is a fundamental and dynamic process that is essential for the maintenance of ion and water balance, especially when fish migrate between fresh water (FW) and sea water (SW) environments. The European eel has proved to be an excellent model species to study the molecular and physiological adaptations associated with this osmoregulatory plasticity. The life cycle of the European eel includes two migratory periods, the second being the migration of FW eels back to the Sargasso Sea for reproduction. Various anatomical and physiological changes allow the successful transition to SW. The aim of this study was to use a microarray approach to screen the osmoregulatory tissues of the eel for changes in gene expression following acclimation to SW. Tissues were sampled from fish at selected intervals over a 5-mo period following FW/SW transfer, and RNA was isolated. Suppressive subtractive hybridization was used for enrichment of differentially expressed genes. Microarrays comprising 6,144 cDNAs from brain, gill, intestine, and kidney libraries were hybridized with appropriate targets and analyzed; 229 differentially expressed clones with unique sequences were identified. These clones represented the sequences for 95 known genes, with the remaining sequences (59%) being unknown. The results of the microarray analysis were validated by quantification of 28 differentially expressed genes by Northern blotting. A number of the differentially expressed genes were already known to be involved in osmoregulation, but the functional roles of many others, not normally associated with ion or water transport, remain to be characterized.

A study of the electrodeposition ͑ED͒ of silicon from silicon halides in a nonaqueous electrolyte solution is described. In spite of the use of nonoxidizing electrolytes, the electrodeposits usually become oxidized on air exposure. In similarity to other work, it is postulated that this is due to the deposit being formed with a spongy or honeycombed morphology. However, secondary neutral mass spectrometry ͑SNMS͒ and X-ray diffraction ͑XRD͒ analyses show that a region of the deposit close to the substrate has sufficient purity for semiconductor properties. This is verified by observing diode behavior at junctions of the deposited layers and mercury contacts. For deposition on crystal silicon substrates, it proved possible to form a p-doped ED-Si/c-Si heterojunction which showed a solar cell conversion efficiency of 1.8%. This is probably the first photovoltaic cell which uses electrodeposited silicon in a heterojunction.

The state-of-the-art in depth-averaged mathematical modelling of 3-D coastal morphology is described for the medium-term morphodynamic model type, in which constituent models of waves, currents and sediment transport based on first physical principles are linked together to describe the time-evolution of the bed topography. Various aspects of the combined system of equations are discussed, such as its mathematical character, its inherent stability and its equilibrium state. The results of an intercomparison of different models are shown for two test cases and the potentials and limitations of the model concept are discussed.

SummaryWe retrospectively compared the changes in serum albumin concentration and colloid osmotic pressure between survivors and nonsurvivors of prolonged (Ն7 days) critical illness over a 2-year period from 1 July 1995. All patients had serum albumin measured daily, and colloid osmotic pressure measured 5 days a week, throughout their ICU admission. (survivors); p < 0.01]. Analysis of colloid osmotic pressure results showed no difference between the groups in mean, minimum or recovery mean. Regression analysis of mean colloid osmotic pressure and albumin revealed that albumin only contributed 17% of the colloid osmotic pressure in these patients. The similar decrease in albumin in nonsurvivors and survivors may reflect the acute inflammatory response and/or haemodilution. However, survivors showed an ability to increase serum albumin concentrations, possibly owing to resumption of synthesis. The colloid osmotic pressure varied little between or within either group of patients, possibly because of the use of artificial colloids. There was no relationship between death and colloid osmotic pressure.