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Abstract. Chlorella sp. is a microalgae that potential for food supplement, pharmaceuticals, animal feed, aqua culture and cosmetics. Chlorella sp. commonly growth in sea water. Indonesia as a producer of tofu generated more liquid waste. Nutrient that contained in the tofu wastewater are very useful for the production of microalgae. Cultivation carried out for 7 days at different percent volume of tofu liquid waste showed that the more volume of tofu liquid waste make them longer process decipherment of polymer compounds in the waste, that's make the growth rate of Chlorella sp. are slowness. Variable of10%V has the fastest growth rate. While, 90% v/v variable has the highest concentration of algae. It shows that Chlorella sp. better to grows in tofu wastewater than seawater.

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Glycerol (C3H8O3) is a polyhydric alcohol compound with three hydroxyl groups in one molecule, polar and viscous in nature. Glycerol can be obtained through the transesterification process in the biodiesel industry, the saponification process in the soap industry and the hydrolysis process in the fatty acid industry. The research method follows two partss, which is catalyst synthesis and steam reforming process. The zeolite catalyst was characterized using X-Ray Diffraction (XRD) to determine crystal characteristic, Scanning Electron Microscopy (SEM) to identify the morphology of the catalyst, and surface area by using BET method. Zeolite catalyst have analcime diffraction pattern Performance of zeolite catalyst was evaluated trough glycerol steam reforming process. The biohydrogen yield increased up to 47% by using zeolite catalyst. Reaction temperature is a parameter that affected to the biohydrogen yield.