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SUMMARY'Sulphur granules' of Actinomyces bovis were isolated from a case of bovine actinomycosis. These were examined in ultrathin sections with the electron microscope, in stained sections with the light microscope or in wet mounts before and after chemical extraction with the light and phase microscope. In addition chemical analyses were done on the granules and on organisms grown in vitro. The combined results showed the granule is a mycelial mass of Actinornyces bovis cemented together by a polysaccharide + protein complex and containing about 50 yo calcium phosphate. With the exception of the calcium phosphate, the granule had essentially the same composition as organisms grown in vitro. It is concluded that the 'clubs' of the granule represent normal hyphae encapsulated with the same polysaccharide + protein complex, that the basic structure of the granule represents the organism itself or products formed by it and that the mycelial mass is mineralized by calcium phosphate derived from the host as a result of phosphatase activity of the host and organism.

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VACCINIA VIRUSand reinjected a few minutes later without difficulty. The newborn mouse easily tolerates the removal of .025 ml of blood or the injection of .05 ml of cell suspension.There are several advantages that this technic provides over the intravenous route. One person can perform the operations without the aid of an assistant, and the inexperienced injector can become quite proficient after the first few attempts. Because of the size of the heart and the transparency of the skin, no magnification is required as with intravenous PARTICLE COUNTS 409 injections. There are no post-injection hematomas, and there is no need to apply pressure after withdrawal of the needle.