Introduction Enzymes are biocatalysts which are synthesized by living cells. chemical reaction. Many bacteria, fungi and higher plants are known to produce pectinolytic enzyme called pectinase, that breakdown pectin, a polysaccharide substrate that is found in the cell wall of plants. One of the most important and widely used commercial pectinase by Henri Braconnot (1825). Pectin is the jelly like matrix structural polysaccharide found in primary cell wall and middle lamella of fruits applications in the production of oligogalacturonides as functional food components [2]. Bacillus cereus is an endemic, soil dwelling, Gram-positive, rod-shaped, beta hemolytic bacterium. Bacillus sp was discovered by Cohn and Koch in the 19 th Bacillus cereus group is composed of B. anthracis, B. cereus, B. mycoides, B. pseudomycoides, B. thuringiensis and B. weihenstephanensis capacity of selected Bacillus sp to produce and secrete large quantities (20-25 g/L) of extracellular enzymes has placed them among the most important industrial enzyme producers [5]. Pectic substances are widely distributed in fruits and vegetables (10-30% in turnips peels of orange and in pulps of tomato, pineapple and lemon); hence they form important natural substrates for pectinases. Pectinases are mainly used Material and Methods Materials