After intravenous injection of glucose, 750 mg/kg, together with a tracer dose of [U-14C] glucose, into fasted, adult white rats, the following percentages of the administered dose were found in whole organs and tissues : 1. after five minutes : skeletal muscle 30.3, skin 28.1, blood 13.1, adipose tissue 10.7, liver 8.9. 2. Forty minutes after injection the corresponding values were: 35.0, 11.1, 5.0, 4.6 and 9.4%. Expired 1~CO~ was negligible after five minutes: after 40 min it comprised 8% of the total dose administered.-After intragastric administration of 1500 mg/kg of glucose given with a tracer dose of [U-14C] glucose under the same experimental conditions, the alimentary tract contained, after 15, 90 and 180 rain, 60.5, 14.8 and 8.4% respectively of the total x4C dose given. At these times the liver contained 2.9, 10.7 and 15.0%; skin contained 7.5, 7.1 and 5.4%; adipose tissue 2.0, 3.8 and 3.5%, and expired 14CO2 0.4, 11.8 and 31.3% respectively. Details of the uptake of ~4C glucose by other organs and tissues are given, and a balance sheet for the injected material is attempted. Contribution de diffgrents organes et tissus du rat d l'assimilation du glucose R~sumd. Aprbs l'injection intraveineuse de glucose (750 mg/kg) en mSme temps qu'une dose traceuse de U-14C-glucose, ~ des rats blancs adultes ~ jeun, los pourcentages suivants de la dose administrde ont dt6 retrouvds dans tousles organes et tissus: 1. aprbs cinq minutes: muscle squelettiquc 30.3, peau 28.1, sang 13.1, tissu adipeux 10.7, foie 8.9.2. Quarante minutes aprbs l'injection, les valeurs correspondantes 6taient los SUlvantes: 35.0, 11.1, 5.0, 4.6 et 9.4%. Le 14CO2 ddgagd dtait ndgligeable apr~s 5 rain: aprbs 40 rain il reprdsentait 8% de la dose totale administrde.-Aprbs l'administration intragastrique de 1500 mg/kg de glucose, donnd en mSme temps qu'une dose traeeuse de U-laC-glucose dans los mSmes conditions expdrimentMes, le tube digestif contenair aubout de 15, 90 et 180 rain, 60.5, 14.8 et 8.4% respectivcment de la dose totale de 140 administrde. A cos moments lk le foie contenait respectivement 2.9, 10.7 et 15.0%; la peau contenait 7.5, 7.1 et 5.4%; le tissu adipeux 2.0, 3.8 et 3.5%, et le laCO2 ddgagd dtait de 0.4, 11.8 et 31.3%. On donne des ddtails sur la captation du 14C-glucose par d'autres organes et tissus, et on essaye de dresser un bilan du produit injectd.

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SUMMARY A fixed dose of glucose, irrespective of body weight, used for i.v. glucose tolerance tests in rats with hypothalamic obesity and in non-obese control rats caused almost identical blood glucose increments in both kinds of rats, and the K values (coefficients of glucose assimilation) were not significantly different. Glucose doses proportional to body weight caused much larger increments of blood glucose in the obese animals but the K value was not significantly different from that for the controls. On the other hand, the insulin sensitivity of obese rats was found to be less than that of control rats when a fixed dose of the hormone was given. When the insulin dosage was proportional to body weight, the fall of fasting blood glucose produced in the obese animals was not significantly different from that in the control group. In vitro, the diaphragm muscle of the obese animals was insensitive to the action of insulin as compared with controls. There was no evidence of insulin insensitivity of the obese animals' adipose tissue; the very low metabolic activity of the adipose tissue of both the control and obese animals may have been responsible. The interpretation of these findings is discussed in the light of similar work in human obesity.

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