9. The spectra of free and antibody-bound DNP lysine differ in 1M propionic acid in a manner similar to that in phosphate-NaC1 buffer, pH 7.4 (L. Wofsy, Ph.D. thesis, Yale University, 1961). The absorption maximum of the free hapten in the propionic acid at 362 m/j is shifted to 378 m/t on binding to anti-DNP and Emax decreases from 1.44 X 104 to 1.04 X 104. Extinction coefficients for the bound hapten were obtained by direct titration of a solution of anti-DNP in propionic acid with DNP lysine in antibody excess. to suggest a specific association of B and A chains of horse antibody globulin. However, the results are inconclusive, since the amount of activity recovered after separation of the chains was only about 2 percent of the original. 15.The first recognized natural transmission of a simian malaria to man occurred accidentally in 1960 with the vivax-like Plasmodium cynomolgi bastianellii as reported by Eyles, Coatney and Getz (1). In 1961 Coatney et al.(2) and Schmidt et al. (3) showed that an old laboratory strain of P. cynomolgi could also be transmitted to man by mosquito bite.Recently we have been able to transmit to a man a second species of simian malaria, Plasmodium brasilianum. This was accomplished by the bites of infected Anopheles freeborni mosquitoes which had been allowed to feed on a spider monkey, Ateles geofjroyi geoffroyi, with an infection acquired naturally in Panama. This quartan parasite of New World monkeys was originally described by Gonder and von Berenberg-Gossler (4) from the white or bald oukari, Brachyurus calvus. Clark (5) 4. G. M. Edelman, B. . 9. The spectra of free and antibody-bound DNP lysine differ in 1M propionic acid in a manner similar to that in phosphate-NaC1 buffer, pH 7.4 (L. Wofsy, Ph.D. thesis, Yale University, 1961). The absorption maximum of the free hapten in the propionic acid at 362 m/j is shifted to 378 m/t on binding to anti-DNP and Emax decreases from 1.44 X 104 to 1.04 X 104. Extinction coefficients for the bound hapten were obtained by direct titration of a solution of anti-DNP in propionic acid with DNP lysine in antibody excess. to suggest a specific association of B and A chains of horse antibody globulin. However, the results are inconclusive, since the amount of activity recovered after separation of the chains was only about 2 percent of the original. 15.

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