Xanthonol, a novel dimeric xanthone, was isolated from a fermentation broth of a non-sporulating fungal species using Sephadex LH20 followed by HPLC and the structure elucidated by spectral analysis. Xanthonol exhibited insecticidal and anthelmintic activities against larvae of Lucilia sericata, Aedes aegypti, and Haemonchus contortus with LD 90 of 33, 8, and 50 mg/ml, respectively.

Mellamide, a novel indole amide, was isolated from a fermentation of Aspergillus melleus using silica gel and high-performance liquid chromatographic methods. This allowed its separation from three known antiparasitic compounds (ochratoxin A, viomellin and xanthomegnin) also present in the potent extract. The structure was elucidated by (1)H, (13)C, COSY, DEPT, HMQC and HMBC NMR experiments. HR-FTMS aided in the molecular weight and formula determination. Mellamide showed in vitro insecticidal activity in bioassays against larvae of Lucilia sericata and Aedes egypti with LD(90) of 1,000 and 50 micro g/ml, respectively.

Isolation and Insecticidal/Anthelmintic Activity of Xanthonol, a Novel Bis-Xanthone, from a Non-Sporulating Fungal Species. -Xanthonol (I), a new dimeric xanthone, exhibits insecticidal and anthelmintic activities. -(ONDEYKA*, J. G.; DOMBROWSKI, A. W.; POLISHOOK, J. P.; FELCETTO, T.; SHOOP, W. L.; GUAN, Z.; SINGH, S. B.; J. Antibiot. 59 (2006) 5, 288-292; Merck Res. Lab., Merck & Co., Inc., Rahway, NJ 07065, USA; Eng.) -M. Bohle 48-217