O levantamento da qualidade de água pode ser um indicador que auxilia o diagnóstico ambiental de recursos hídricos necessários em ambiente periurbano. O presente trabalho teve como objetivo avaliar a qualidade microbiológica da água de nascente localizada no córrego Siriema (setor intermediário) no município de Jandaia do Sul-Paraná. Em campo, foi coletado amostra de água da nascente na margem direita (23º 35' 18" S e 51º 38' "22 O), situada na região próxima à rua. A análise

The present study evaluated the isolated and combined effect of total ammonia and nitrite on Macrobrachium amazonicum adults through the median lethal concentration (LC5096h), histological analysis of gills and total and differential haemolymph cell counts. Prawns (n = 640; 9.8 ± 0.9 cm and 8.22 ± 1.29 g) were subjected to an entirely randomised design in a 4 × 4 factorial scheme. Individuals were exposed to total ammonia (0, 18, 36 and 72 mg/L) and nitrite (0, 1, 2 and 4 mg/L) alone and in combination. Interaction between the compounds was determined by summing the additivity of the substances (S) and by calculating the additivity index (AI). The LC5096h of isolated compounds was 27.98 mg/L for total ammonia and 1.87 mg/L for nitrite. Total ammonia and nitrite combined showed an LC5096h of 22.36 and 1.17 mg/L, respectively. The sum of additivity ranged from 2.70 to 1.42 and the AI indicated that the toxicity of the mixture was 1.70 to 0.42 times lower, indicating an antagonistic effect of the compounds. High total haemocyte counts (THC) were observed at the higher concentrations of both compounds at a shorter exposure time. Reduction in THC was observed in the treatments with a longer exposure time. Differential haemocyte counts were similar between treatments. Increases in the extent of regressive damage and organ index (Iorg) values occurred with increasing concentrations of isolated total ammonia and nitrite. Mild to moderate presence of progressive damage was observed at intermediate combined concentrations, which elevated the Iorg values. The interaction of total ammonia and nitrite promoted a reduction in mortality compared to treatment with the isolated compounds. Thus, the results obtained guide decision‐making in production systems, which are currently based on studies where the compounds were evaluated acting in isolation.

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