Cyclin-dependent kinase 2 (CDK2) is a member of a highly conserved family of protein kinases that regulate the eukaryotic cell cycle. The crystal structures of the human CDK2 apoenzyme and its Mg2+ ATP complex have been determined to 2.4 A resolution. The structure is bi-lobate, like that of the cyclic AMP-dependent protein kinase, but contains a unique helix-loop segment that interferes with ATP and protein substrate binding and probably plays a key part in the regulation of all cyclin-dependent kinases.

MEASURING CO IN RESPIRED GASESPco2 are discussed. The most important practical conclusion is that the volume of gas in the bag should not be more than twice the tidal volume; but the exact ratio is not critical.Suitable equipment is described. Practical problems and variations in technique are described in detail.We are grateful to the many people who have told us of their experience and difficulties, and particularly to those who have suggested modifications and improvements. Dr.

No abstract

No abstract

No abstract