INTRODUCTION Interprofessional education and collaborative practice is emerging at the forefront of health professions education. The Commission on Accreditation of Athletic Training Education recently added interprofessional education and interprofessional collaborative practice to the accreditation standards. In order to facilitate interprofessional education experiences and produce collaborative, team-based care athletic trainers, the development of preceptors in interprofessional education and collaboration is vital. The purpose of this study was to examine the effectiveness of an asynchronous online professional development module designed for preceptors who are part of athletic training programs, to educate them in an attempt to promote interprofessional education and collaboration in the clinical setting for athletic training students. METHODS A pre-experimental retrospective pretest-posttest survey design was used to measure the effectiveness of an online asynchronous professional development module focused on interprofessional education and collaboration. The population for this study was healthcare practitioners who serve as preceptors in accredited athletic training programs. RESULTS Statistical significance was found between the pretest and posttest scores for athletic training preceptors on the ISVS-21.CONCLUSION These findings provide evidence that a 10-minute asynchronous online professional development module is effective in influencing preceptors' beliefs, behaviors, and attitudes toward interprofessional collaboration.