We report for the first time the chemical composition of volatile components (VOCs) of two subspecies, D. hirsuta subsp. hirsuta and D. hirsuta subsp. nepalensis, of the liverwort Dumortiera hirsuta from Panama by using headspace-solid phase microextraction-gas chromatography-mass spectrometry in order to assess distinguishing markers between the two species. Forty VOCs were identified in total for both subspecies. Of these, 34 are reported for the first time in D. hirsuta. Furthermore, both subspecies showed clear differences in the type and amount of VOCs. The major compounds in D. hirsuta subsp. hirsuta were α-gurjunene, β-selinene, α-guaiene, α-humulene and β-caryophyllene; while in D. hirsuta subsp. nepalensis were ledene, α-gurjunene, β-caryophyllene and α-guaiene, respectively. Two oxygenated sesquiterpenes, globulol and nerolidol, could be considered as possible distinguishing chemical markers between these two subspecies. We conclude that both morphotypes of D. hirsuta are chemically different.

We report for the first time the chemical profiling of volatile organic compounds (VOCs) of gametophyte and sporophyte life stages of Leiosporoceros dussii, from Panama by using headspace-solid phase microextraction-gas chromatography-mass spectrometry in order to assess distinguishing chemical markers between the male and female gametophytes, and sporophytes of this hornwort. A total of 27 VOCs were identified in L. dussii. Furthermore, the gametophyte and sporophyte showed clear differences in the type and amount of VOCs. The main constituents of L. dussii female thalli were menthacamphor (17.8%), hexanol (12.3%), and menthyl acetate (12.3%), while the major compounds of the male thalli were hexanol (25.3%), β-ionone (21.1%), benzeneacetaldehyde (17.6%), and β-cyclocitral (14.0%). The main VOCs of the sporophytes were hexanal (19.3%), β-cyclocitral (17.6%), 2-nonenal (15.8%), hexanol (12.5%), and β-ionone (10.2%). Unique compounds found in the female thalli were 3-pentanone, 3-octenol, nonanol, estragole, and menthyl acetate, and in the male thalli were methyl heptenone, nonanal, neoisomenthol, and bornyl acetate. Isomenthol, thymol, isomenthol acetate, and β-methylnaphthalene were only found in the sporophyte. The characteristic VOCs identified in L. dussii suggest a difference between the chemical constituents of L. dussii and other hornworts species. The presence of simple VOCs when compared with compounds previously characterized in another hornwort genera may support the distinct genetic nature of this species.

Summary: Morphology and distribution of Dolotortula mniifolia and Trachyphyllum dusenii (Bryophyta) in Panama. The genus Dolotortula, and the species D. mniifolia, are reported for the first time for Panama. Branched stems and multistratose yellow leaf border with 5-6 rows of stereids (in cross section) are described for the first time for the Central American populations of D. miniifolia. The morphology and geographical distribution of Trachyphyllum and the species T. dusenii, recently reported for Panama, are revised and updated. Perichaetial leaves with weakly serrulate margins are described for the first time for T. dusenii. The report of this species in Panama represents the northernmost distribution known for the species in tropical America and includes the only populations known for the species in the pacific coast. An updated geographical distribution for both species is presented.Key words: Central America, Dolotortula, morphology, mosses, Neotropic, Panama, Trachyphyllum.Resumen: El género Dolotortula y la especie D. mniifolia, se registran por primera vez para Panamá. Se describen por primera vez para las poblaciones de Centroamérica de D. mniifolia tallos ramificados y borde de la hoja de color amarillo, éste en corte transversal, con 5-6 hileras de células de paredes engrosadas. Se detalla y amplía la morfología y distribución geográfica de Trachyphyllum y la especie T. dusenii, recientemente reportados para Panamá. Se reportan por primera vez hojas periqueciales débilmente serruladas para esta especie. La distribución de esta especie en Panamá es la más septentrional conocida para la América tropical, e incluye las únicas poblaciones del género conocidas para la vertiente del Pacífico. Se incluyen mapas con la distribución geográfica actualizada para las dos especies.