RESUMEN.En esta investigación se evaluó el efecto de la exposición de cobre en la capacidad de bioacumulacion de metal en células de Euglena gracilis. La acumulación de metal medida con la técnica de fluorescencia de rayos X (XRF) mostró que la acumulación de Cu +2 en E. gracilis fue dosis dependiente y se incrementó significativamente en las células tratadas con 0.4 y 0.8 mM de Cu +2 con respecto al control. No se descarta la presencia de una estrategia de acumulación en E. gracilis que podría involucrar la participación de una serie de múltiples procesos, como producción de vacuolas. Futuros estudios al respecto deberán orientarse a evaluar la capacidad de bioacumulación de E. gracilis para su aplicación en programas de biorremediación de sistemas acuáticos. Palabras clave: Euglena gracilis, XRF, cobre, contaminación, tolerancia, ecotoxicología. Assessment of copper bioaccumulation in Euglena gracilisby X-Ray fluorescence technique ABSTRACT. The effect of the exposure to copper in metal bioaccumulation capacity in cells from Euglena gracilis was evaluated in this study. Metal accumulation measured with the X-ray fluorescence (XRF) technique showed that the accumulation of Cu +2 in E. gracilis was dose dependent and was significantly increased in cells treated with 0.4 and 0.8 mM of Cu +2 with respect to control. The presence of an accumulation strategy in E. gracilis that could involve the participation of a number of multiple processes, such as production of vacuoles, is not discarded. Future studies should be oriented to evaluate the potential for bioaccumulation of E. gracilis to be applied in bioremediation of aquatic systems programs.
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