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Twenty-four near-isogenic barley lines, with a cv. 'Pallas' background, carrying different mildew resistance genes were subjected in 1987,1989 and 1990 to natural infection by the pathogen at several different and contrasting Spanish sites in order to study its virulence. The virulence genes proved to be geographically grouped into three regions: western (Valladolid), southern (Sevilla) and northern and northeastern (Navarra, Lleida and Girona). The mildew population of Lleida was more variable when compared with Navarra and Valladolid. Overall, the most effective resistance genes were: Ml-al3 -\-Ml-(Ru3), mlo and Ml-(1402).

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Se concluye que la evaluación de las semillas mostró variabilidad en calidad.