Purpose: Quantify the progressive decrease of the cellular viability of rabbit meniscus preserved for transplants over a 30 day period at different freezing temperatures. Methods: 180 menisci were removed from 45 rabbits. Menisci were frozen from 2 to 30 days at -7.2°Celsius -21.4°Celsius and -73°Celsius. Four menisci from each temperature were thawed every two days and the number of present cells was quantified. RESUMOObjetivo: Quantificar a diminuição progressiva da viabilidade celular de meniscos de coelhos preservados para transplantes durante trinta dias em três temperaturas diferentes de congelamento. Métodos: Retirou-se 180 meniscos de 45 coelhos. Os meniscos foram congelados, de dois até trinta dias, a -7,2°Celsius -21,4°Celsius e -73°Celsius. A cada dois dias, de cada temperatura, foram descongelados quatro meniscos e quantificou-se o número de células presentes. Resultados: No 14º dia de congelamento a -7,2°Celsius, a média de células viáveis foi de 92,38%. Entretanto, a partir do 16º dia observou-se uma redução significante de 12% (p=0,001), comparando-se com a média no 14° dia. A média da viabilidade celular, nas temperaturas -21,4°Celsius e -73ºCelsius, até 16º dia apresentou comportamento estatisticamente semelhante. A partir do 18º dia na temperatura de -21,4°Celsius a redução do número de células foi significante (p<0,001), especialmente do 28°( 54,5%) para o 30° dia (30%) de congelamento. O número de células viáveis a -73°Celsius mostrou uma redução não significante (p=1,000) de 2,3% no número de células viáveis do 28° (40,2%) ao 30° dia (37,9%). Conclusão: A quantidade de células de meniscos viáveis é semelhante até o décimo quarto dia de preservação, em qualquer das três temperaturas testadas. No entanto, à partir deste período até o trigésimo dia a quantidade de células viáveis é menor nas temperaturas mais baixas. Descritores: Congelamento. Meniscos. Transplante. Coelhos.

Objective: to identify the prevalence and factors associated with falls in the elderly population living in rural areas. Method: this is a cross-sectional study, carried out in 2014, with 820 older adults recorded in the Family Health Strategy (Estratégia Saúde da Família). The association between the reporting of falls in 12 months and their associated factors was verified by the Chi-square and Fischer’s exact tests and by multivariate logistic regression analysis. Results: the majority of the sample was female (56.1%), white (90.2%) and aged 60-69 years (54.9%). The prevalence of falls was 27.9%, and being female, hypertensive and diabetic was associated to falls. Conclusion: it is the responsibility of health professionals to have a closer look at the elderly who have these chronic diseases, especially within the scope of the Family Health Strategy, which works longitudinally with these patients, in addition to improving nursing care aimed at this population.

Synthetic surgical adhesives derived from cyanoacrylate acid promoted cortical to medullary bone necrosis bone in both groups.

Objective: To evaluate the ability of fibrin adhesive in promoting the meniscus fixation within two, four and eight weeks compared to the conventional soft-tissue suture technique. Materials and Methods: 36 right medial menisci of rabbits preserved at negative 73° Celsius for 30 days were transplanted to animals of the same sample and fixed with soft-tissue suture or fibrin glue. After 2, 4 or 8 weeks, the appearance of the menisci and the quality of fixation were macroscopically checked and evaluated by a scoring system. The findings were subjected to the statistical study of variance analysis (p ≤ 0.05%). Results: The deep-frozen meniscus preservation maintained the integrity of the meniscus transplant, and, macroscopically, there was no significant reduction of the length of the meniscus in all post-transplant periods (p = 0.015). The menisci fixed with fibrin showed slight changes in color and surface roughness. There were no signs of rejection or infection in both groups. Suture fixation scoring was superior (p = 0.015) in all periods (80% of total fixation) as compared to the setting promoted by fibrin (20% of total fixation). Conclusion: The homologous transplantation of the meniscus of rabbits experienced various degrees of integration to the knee according to the fixation method; the surgical soft tissues suturing technique was shown to be superior in the evaluation of scores compared to the fixation with fibrin adhesive.

No abstract