Abstract. This paper discusses the effects of pH and concentration on the capability of E. coli ATCC29522 and S. epidermidis RP62A biofilm with bentonite in removing divalent copper, nickel and lead from wastewater. Batch adsorption study at laboratory scale was utilized to evaluate the potential use of bacterial biomass (E. coli ATCC29522 and S. epidermidis RP62A) aided with geosynthetic clay (bentonite) for the removal of Cu 2+, Ni 2+ and Pb 2+ . Results revealed that removal of Cu 2+ , Ni 2+ and Pb 2+ by both types of organisms supported with bentonite were high in the first 4 hours of the experiment. This illustrates that the binding site on that particular time was abundant. Hence, the removal rate was evident at high concentration depicting the line adsorption equilibrium. It also revealed that S. epidermidis RP62A supported with bentonite had the highest affinity to Copper and Lead with Qm = 277.7 mg/g and 5.0075 mg/g, respectively. While E. coli ATCC 29522 had the highest affinity to Nickel (Qm= 58.82 mg/g). Hence, the sorption of Cu 2+, Ni 2+ and Pb 2+ onto E. coli ATCC29522 and S. epidermidis RP62A biofilm supported with bentonite clay occurred through monolayer chemisorption on the homogeneous surface of E. coli ATCC29522 and S. epidermidis RP62A biofilm with bentonite clay. Batch kinetics studies revealed that the sorption of Cu 2+ , Ni 2+ and Pb 2+ onto E. coli ATCC29522 and S. epidermidis RP62A biofilm supported with bentonite clay was well described by a pseudo-second-order equation model of type 1 (R2 = 0.9999), which implies that chemisorption is the rate limiting step.