An ultrasonic micronebulizer Interface for effective nebullzation at 2-20 juL/mln liquid was developed to allow the determination of nonvolatile analytes by microbore HPLC with virtually unmodified gas chromatographic detectors. A detectability of 50 pg/s phosphorus was obtained with a dynamic range of 3 decades and acceptable peak broadening using a flame photometric detector (FPD) as a detector. Solvent compatibility appears mainly determined by the choice of chromatographic detector. Dual-wavelength operation can Improve the slgnal-to-noise ratio by a factor of 5, Improve the precision from 6 to 2%, and eliminate base-line shifts. The utility of the mlcronebullzer-FPD as a selective HPLC detector Is demonstrated In the analysis of phospholipids and sugar phosphates.

No abstract