toxin by the usual method indicated a degree of adsorption in excess of 94%. The h4.L.D. of the original toxin was 0.0035 cc. while that of the adsorbed toxin was less than 0.0037 cc.Nitrogen determinations (by micro-Kjeldahl) indicated that the nitrogen loss sustained during the process of purification was in excess of 90%.Tetanospasmin and staphylolysin were similarly adsorbed to and eluted from the magnesium precipitate. Tetanolysin, however, was destroyed.
C~nclzm'ons:( 1 ) Magnesium hydroxide in colloidal suspension adsorbs diphtheria toxin which can be eluted without destruction of the toxin. (2) The adsorption is approximately 94% complete.( 3 ) The nitrogen loss per M.L.D. sustained during the process is in excess of 90%. (4) The method has been used with success in the adsorption of tetanospasmin and staphylolysin but not with tetanolysin and indicates possibilities for the purification of other Incterial products.Thanks are due Prof. E. E. Ecker for many helpful suggestions and criticisme.
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