This study set out to evaluate the phytochemical composition of Erythrophleum suaveolens stem bark extract and its fungicidal attributes towards Aspergillus flavus isolated from Zea mays. The stem bark extract was obtained using soxhlet extraction method with methanol, chloroform, and water as solvents. Qualitative and quantitative phytochemical analysis was then carried out to determine the concentration of phytochemicals present in the stem bark extracts, this were the tannin, flavonoid, saponin and phenol with a concentration of 2.47, 1.328, 2.72 and 0.838 mg/g respectively. For the antifungal effect, different crude concentrations of the stem bark extracts were then added to agar wells within Aspergillus flavus swabbed in a potato dextrose agar plate for 2hrs at room temperature and allowed for diffusion to take place. After 48hrs, the cultured samples were examined for zones of inhibition. The water and chloroform extracts had an inhibitory diameter zone of 10 and 2 mm respectively. The utilization of plant extract of Erythrophleum suaveolens could form an alternative to synthetic fungicides/pesticides used in the management of pests and fungi diseases. Significant implication from the result was that, water extract of the plant had higher antifungal potency towards A. flavus isolated from Zea mays
This study is to evaluate the biological disinfectant ability of Aloe vera (under different conditions and parameters) on coliform isolates from leachate that emanate from a dumpsite.Leachate samplewas subjected to a presumptive test to evaluate the presence of coliforms in them; the positive samples were further subjected to a confirmatory test to identify the thermo-tolerant nature of the coliform. The coliforms were thermo-tolerant Escherichia coli (FTE) and Thermo-tolerant coliform (non E. coli) (FTC) Disinfectant efficacies of Aloe vera (with various condition and parameters) were evaluated on the isolated microorganisms from the leachate samples, using the disc diffusion method. The Aloe vera showed that concentration of the disinfectant matters in its efficacy with 5% storage concentration showing high level of zone of inhibition with the isolated microorganisms. Also the Aloe vera was more effective at a lower pH and storage temperatures of 0, 25 and 35oC.
This study set out to evaluate the phytochemical composition of Erythrophleum suaveolens leaves extract and its fungicidal attributes on Aspergillus flavus isolated from maize seeds. The leaves of E. suaveolens were collected from Gwagalada, Abuja, Nigeria, and subjected to soxhlet extraction with methanol and chloroform as solvents to determine the bioactive compounds in the leaves compared to water crude extract. Different crude concentrations of the plant extracts were added for the antifungal effect into wells within Aspergillus flavus swabbed in a potato dextrose agar plate to diffuse at room temperature for 2hrs and the plates were incubated at 28oC for 48hrs before measuring the diameter of the inhibition zones. Tannins, alkaloid, phenols, Triterpenoids, saponin, and flavonoid were present in the plant with tannin, flavonoid, saponin, alkaloid, and phenol having a concentration of 2.49, 1.448, 2.69, 0.505 and 0.366 mg/g respectively. The water, chloroform, and methanol extracts had an inhibitory diameter zone of 10 ± 0.58, 3 ± 0.58, and 0 mm, respectively at p<0.05. This study showed the presence of some bioactive compounds in E. suaveolens. Furthermore, water extract of the plant had higher antifungal potency on A. flavus isolated from maize seeds.
Food safety and security well implemented could help in making more crops available for consumption. Maize seed is a crop well known to be attacked by fungi such as Aspergillus flavus and reduce its nutrients. This study intends to isolate and morphologically identify the Aspergillus flavus from maize seeds from Abuja, Nigeria. The experimental design was complete randomized design involving untreated yellow (Y) and white (W) maize seeds from7 locations in Abuja, Nigeria. Pure culture of fungal isolate was prepared using Potato Dextrose Agar (PDA) and Sabouraud Dextrose Agar (SDA). Isolates obtained were characterized and identified on the basis of their colonial and morphological characteristics which include macroscopic and microscopic examinations. All maize seeds from the Abaji had no fungi incidences in both SDA and PDA, while all maize seeds from the experimental field show fungi incidences in both SDA and PDA. On SDA, the maize color yellow and white had F (2, 6) static values of 7.083 and 0.212 at p=0.129 and 0.941 respectively. For the PDA, white maize seeds and yellow maize seeds from all the locations had F (2, 6) static values of 0.377 and 0.521 at p=0.850 and 0.773. Aspergillus flavus was isolated in this study. The maize seeds from Kuje district and the experimental field 2 show a high incidence records. All growth of the Aspergillus in the two media used were not significant at p>0.05.
Anthropologic activities in our environment had been continuously associated with the release of nitrate a contaminant that has been linked with some dangerous health effects. This study assessed the concentration and health risk of nitrate in groundwater used by residents around a dumpsite. For this study groundwater samples were obtained randomly from 12 sampling sites near the Solos dumpsite at Igando, Alimosho local government area, Lagos, Nigeria. The water samples were analyzed for nitrate and some other physic-chemical parameters. The water quality index of the water samples was calculated to determine the suitability for consumption purposes. The non-carcinogenic hazard health risk associated with the nitrate level in the water samples was also assessed. The relationship between the nitrate in the water samples and the drinking water quality index (DWQI) was a positive one with r= 0.21 at p=0.517. The hazard index for ingestion (oral) route (HIoral) range were 0.024-0.962, 0.028-1.136 and 0.033-1.3 for male, female and children respectively. The dermal hazard index (HIdermal) shows range of 0.001-0.026 for male, 0.001-0.027 for female and 0.002-0,071 for children. Sample 6 had Total hazard index (HItotal) greater than 1for both female adult and children as 1.163 and 1.371 respectively. All water samples on this study had the presence of nitrate that were positive correlated with water quality index and only one of the samples nitrate concentration was associated with high health non-carcinogenic risk effect especially in children and women.
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