| During an outbreak of Foot and Mouth Disease (FMD) blood samples were collected from clinically diseased (n=10) and healthy (n=10) cattle and analysed for various hemato-biochemical parameters. Results indicated a significant increase in packed cell volume (PCV) (28.6 ± 0.98%, P=0.036), lymphocytes (70.2 ± 6.20%, P=0.0132), monocytes (8.2 ± 1.08%, P=0.0132) and serum glucose (104.2 ± 15.59 mg/dL, P=0.0053) whereas a significant decrease in the concentration of hemoglobin (7.17 ± 0.07 gm/dL, P=0.0004), basophils (0.3 ± 0.16%, P=0.0241), eosinophils (16.5 ± 1.83%, P=0.0097), calcium (8 ± 0.41 mg/dL, P=0.0125) and total protein (6.2 ± 0.19 mg/dL, P=0.0058) was recorded in FMD infected cattle. Non-significant (P >0.05) difference was noted between healthy and FMD infected animals with respect to total erythrocyte count (TEC) (P=0.6066), total leukocyte count (TLC) (P=0.3169), neutrophils (P=0.2358) and serum inorganic phosphorous (P=0.2507). It was concluded that FMD had major impact on hemato-biochemical profile of affected animals.