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Owing to its environment-friendly, biocompatible, non-toxic nature of nanoparticles attained from green plant extract, it has remarkable significance in the field of nanotechnology towards effective biomedical application. This work reports the synthesis of copper oxide nanoparticles by means of Cissus quadrangularis and Piper betle via greensynthesis route. The medicinal leaf extracts possessing phytochemicals such as alkaloids and polyphenols act as a reducing agent in the current synthesis. The characterization of functional groups of CuO NPs by two different leaf extracts was investigated by FTIR analysis which measures reduction and stabilization of CuO NPs. X-ray diffraction confirms the orthorhombic structure with an average crystallite size of about 32.54 and 32.09 nm. The irregular spherical-shaped morphology of the samples is evidenced from scanning electron microscopy analysis. The transmission electron microscopy images demonstrated 50 nm sized prepared CuO nanoparticles. The bioactivity of the prepared sample is confirmed from antibacterial studies and zeta potential.This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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