BackgroundMedicinal plants are becoming more popular in the treatment of various diseases because of the adverse effects of the current therapy, especially antioxidant plant components such as phenols and flavonoids have a protective role against oxidative stress-induced degenerative diseases like diabetes. Thus, the purpose of this study was to investigate β-cell protection and antidiabetic activities of Crassocephalum crepidioides (Asteraceae) Benth. S. Moore.MethodThe in-vitro study was conducted by the pancreatic β-cell culture and α-amylase inhibition technique which includes two methods, namely starch-iodine method and 3,5-dinitrosalicylic acid (DNSA) method. On the other hand, the in-vivo study was performed by oral glucose tolerance test (OGTT) method and alloxan-induced diabetes method by using Wistar albino rat. At the end pancreatic specimens were removed and processed for histopathological study.ResultThe plant extract showed significant (*p < 0.05, **p < 0.01) effect on hyperglycemia as compared to standard (Gliclazide) in OGTT. The plant extract showed efficient protection activity of pancreatic β-cell from cell death in INS-1 cell line by significantly reduced (*p < 0.05, **p < 0.01) the levels alloxan-induced apoptosis and intracellular reactive oxygen species (ROS) accumulation. In addition, the plant extract showed a significant (*p < 0.05, **p < 0.01) effect on hyperglycemia by increases in percent of β-cells present in each islet (45% – 60%) compared to the diabetic group.ConclusionThe result showed that C. crepidioides had β-cell protection and antidiabetic activities in pancreatic β-cell culture and Wistar albino rat.Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-017-1697-0) contains supplementary material, which is available to authorized users.

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Aims: Lablab purpureus (Fabaceae) a well-known Bangladeshi vegetable combines a great number of qualities with its adaptability and functional food properties. But still there is no evidance based report on neuropharmacological activities of Lablab purpureus seeds. For this reason, the present study was designed to analyze in vivo CNS effect of methanolic extract of Lablab purpureus seeds. Place and Duration of Study: Department of Pharmacy, USTC between April 2017 and May 2018. Methodology: In this study, the crude seeds extract of Lablab purpureus was evaluated for its sedative and anxiolytic effect using animal behavioral models, such as open field and hole cross tests for its sedative properties and an elevated plus maze (EPM) test for its anxiolytic potential. Results: In open field and hole cross test, the seeds extract of Lablab purpureus showed a dose dependent suppression of motor activity and exploratory behaviour. In EPM test, animals treated with higher dose of methanolic extract showed significant (p<0.01) increased exploration to and time spent in EPM open arms. Conclusion: This study provide in vivo evidence that the methanolic extract of Lablab purpureus has significant sedative and anxiolytic activity. Further studies on active constituent of the extract can provide approaches for therapeutic intervention.

Cefoperazone as a Cefoperazone sodium is a semisynthetic broad-spectrum cephalosporin antibiotic for which is used as a parenteral drug. Cefoperazone is quite effective against a wide variety of gram positive and gram-negative organisms including staphylococcus, Enterobacter, streptococcus, klebsiella and salmonella species. Cefoperazone is widely used in the treatment of Urinary tract infections, pelvic inflammatory diseases, respiratory tract infections etc. The principal purpose of present follow a line of investigation was to explore in vitro communications of Cefoperazone with crucial metal salt furthermore antacid to settle on the bioavailability in view of antimicrobial movement of Cefoperazone following drug metal communications at the pH7.4. Cefoperazone is one of the cephalosporin drugs to is exercised to treat numerous different sorts of infections originated by bacteria. Since the existence of compelling the ligand may influence bioavailability of metal in blood and tissues, for that reason with the intention of study the handy interaction of the Cefoperazone with vital elements in attendance in the body. Cefoperazone has been related with Zn, Mg as an in-vitro examination. Besides, the anti-microbial movement of drug along with the complexes was resolved. It has scrutinized that Cefoperazone interrelates with metal and antacid on the pH 7.4 by plotting various UV spectrophotometric methods. This examine authenticates that there was the interaction between Cefoperazone and metal, antacid which was authenticated by job's plot technique furthermore by antimicrobial exploration. This was authenticated that region of inhibition of Cefoperazone with Metal in addition to antacid condensed from 16mm to 14mm and 13mm correspondingly. The typical Cefoperazone disk also checked in opposition to Staphylococcus aureus.