The development of a particular ecosystem requires water for sustainability of living organisms. Different organic and inorganic components are dissolved in water, as it is universal solvent. The optimum level of different constituents is responsible to maintain respective quality for the suitable growth and development of plants, animals and micro-organisms. The physico-chemical parameters of water samples collected in the year 2018-2019 from Jayakwadi dam were analysed. To understand these parameters is important, as the Jaykwadi dam water is essential for drinking, irrigation and industrial concern of Marathwada region. Therefore, in this paper different physico-chemical parameters viz. Temperature, pH, DO, BOD, Hardness, alkalinity, TDS, turbidity, Chlorides, Phosphates, chlorine, nitrates etc. are studied.