Congenital Zika Syndrome (CZS) has been associated with microcephaly and other central nervous system abnormalities including areas that have been implicated in the control of the lower urinary tract [1] Neurological lower urinary tract dysfunction (NLUTD) is a common condition among patients with CZS and microcephaly [2][1][3][4][5]. But the lack of knowledge that CZS causes NLUTD delays investigation and treatment. This revised version includes changes based on the observations of four-years of experience using our first published protocol [6], the new sequels found in children with CZS, which are cryptorchidism [7] and the neurogenic bowel [5], and related publications [8][9]. This new version includes the vision of the authors, who are from five different institutions in Brazil. They have been working with patients with NLUTD and participate in the development of the new Urological care network for patients with CZS. Our protocol aims to alert health professionals to the relationship between neurological lower urinary tract dysfunction and Congenital Zika Syndrome and to initiate an early investigation to minimize the risks associated with neurological lower urinary tract dysfunction and other genitourinary disorders that may be found in these children. A thorough investigation of these children can reduce the impact of this important sequelae, which is the neurological lower urinary tract dysfunction, reducing comorbidities and consequent impairment of renal function and mitigating the disease burden for patients and families and the Health Systems.